AN IMPROVED LC-MS/MS METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF TRANDOLAPRIL AND VERAPAMIL IN HUMAN PLASMA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2019, Vol 11, Issue 3
Abstract
Objective: A simple, sensitive and rapid LC-MS/MS technique was developed for the quantitation of trandolapril (TDL) and verapamil (VPL) in a biological matrix and validated. Methods: Sample preparation processed by SPE (Solid Phase extraction) on phenomenex cartridge using Ledipasvir as an internal standard. Two drugs were eluted on waters symmetry-RP18 (5µ, 150 mm×4.0 mm) column with the mobile composition of 10 mmol ammonium formate and ACN(acetonitrile) in the ratio of 70:30 %V/V. Detection and quantitation were processed by electrospray ionization in positive ionization mode. Results: The quantification approach was validated in 5-1500 ng/ml linear concentration range for TDL and 1-2000 ng/ml for VPL. The intraday and inter-day precision and accuracy were found to be 0.58% to 5.69% and 93% to 104% for two drugs. The average recoveries for TDL and VPL were found to be 92.9% and 93.5% respectively. Conclusion: The developed work was validated and can be applicable to the routine analysis of TDL and VPL simultaneously in a biological matrix.
Authors and Affiliations
YATHA RAVI, B. RAJKAMAL
Keywords
Related Articles
- EP ID EP565430
- DOI 10.22159/ijpps.2019v11i3.31247
- Views 89
- Downloads 0
ANTIMICROBIAL PROPERTIES OF LEPTADENIA HASTATA (PERS) DECNE LEAVES EXTRACT
Objective: This study was carried out with an objective to investigate the antibacterial potential of five leaf extract of Leptadenia hastata plant on some bacteria.Methods: Leptadenia hastata extracts were evaluated for...
ACUTE DERMAL TOXICITY OF COAL FLY ASH NANOPARTICLES IN VIVO
Objectives: To study the toxicity effects of coal fly ash nanoparticles-induced acute dermal toxicity in Wistar albino rats.Methods: Acute dermal toxicity studies for coal fly ash nanoparticles (CFA-NPs) were conducted i...
DURATION DEPENDENT ANTIAPOPTOTIC EFFICACY OF CAMELLIA SINENSIS (GREEN TEA) ON STREPTOZOTOCIN INDUCED DIABETES LINKED TESTICULAR HYPOFUNCTION IN ALBINO RAT: GENOMIC AND FLOW CYTOMETRIC ASSESSMENT
Objective: The study has been conducted to search out the threshold duration of treatment of ethyl acetate fraction of methanolic extract of leaves of Camellia sinensis (L.) Kuntze at the dose of 100 mg/kg body weight fo...
STROKE OUTCOMES IN NON-DIABETIC, DENOVO DIABETIC AND DIABETIC INDIAN PATIENTS MEASURED BY MODIFIED RANKIN SCALE: AN OBSERVATIONAL STUDY
Objective: Hyperglycemia is a known risk factor which adversely impacts the outcomes in stroke patients compared to patients with normal blood glucose levels. Patients suffering from an acute stroke who are previously no...
IN VITRO DPPH RADICAL SCAVENGING AND ANTI-BACTERIAL ACTIVITY OF OMAN’S CYMBOPOGON
Objective: The objective of this work is to evaluate antioxidant and anti-microbial activity of methanolic extract of Omani Cymbopogan schoenanthus.Methods: Antibacterial activity of mehanolic extract of Cymbopogan was e...