AN IMPROVED RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ASPIRIN, ATORVASTATIN, AND CLOPIDOGREL IN PHARMACEUTICAL FORMULATION USING EXPERIMENTAL DESIGN METHODOLOGY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 11
Abstract
Objective: In this study an improved RP-HPLC method was developed for the simultaneous estimation of Aspirin, Atrovastatin, and Clopidogrel in pharmaceutical dosage form. For improving the separation, an experimental design approach was employed.Methods: Factors–independent variables (Organic modifier, pH of the mobile phase and flow rate) were extracted from the preliminary study and as dependent three responses variables viz. Capacity factor of tR1, resolution between Atorvastatin and internal standard, retention time of tR4 were selected. To improve method development and optimization, Derringer’s desirability function was applied to simultaneously optimize the chosen three responses.Results: The procedure allowed deduction of optimal conditions and the predicted optimum was Acetonitrile: Methanol: 0.1% of Triethylamine (52:05:43, v/v/v), pH of the aqueous phase adjusted at to 3.0 with 10 % ortho phosphoric acid, and the separation was achieved within 8 minutes. The method showed good agreement between the experimental data and predictive value throughout the studied parameter space.Conclusion: The optimized assay condition was validated according to International Conference on Harmonisation (ICH) guidelines to confirm specificity, linearity, accuracy and precision. The proposed validated method was successfully applied for the analysis of commercially available dosage form.Â
Authors and Affiliations
R. Sathiyasundar, K. Valliappan
GUAR GUM: A VERSATILE MATERIAL FOR PHARMACEUTICAL INDUSTRIES
Guar gum (GG) is galactomannan, derived from guar (cyamopsistetragonolobus) kernels which belong to family Leguminosae. The solution of guar gum in water has the highest viscosity amongst all the natural polysaccharide d...
SYNTHESIS, CHARACTERIZATION AND PRELIMINARY ANTIMICROBIAL EVALUATION OF NEW SCHIFF BASES OF AMPICILLIN AND AMOXICILLIN DERIVED FROM ISATIN DERIVATIVES
Objectives: Six different Schiff bases were synthesized from ampicillin and amoxicillin with isatin, 5-bromoisatin, and 5-nitroisatin.Methods: Ampicillin and Amoxicillin are linked directly through their α-amino groups...
POLYMERIC NANOPARTICLES FOR IMPROVED BIOAVAILABILITY OF CILNIDIPINE
Objective: In the present study, we aimed to optimize, characterize and evaluate poly lactic-glycolic acid nanoparticles of cilnidipine for improved permeation across the gastrointestinal tract.Methods: Poly lactic-glyco...
COCONUT MILK MODULATE THE ANTIGENICITY OF ALPHA-LACTALBUMIN IN BALB/C MICE
Objective: The aim of this work was to study the biochemical characteristics of coconut milk and its antigenic effect on the Balb/c mice immunized with α-lactalbumin protein, as well as its consequences on the structure...
ANTITUMOUR ACTIVITY OF MESO-ZEAXANTHINE AND ITS MECHANISM OF ACTION
Objective: To evaluate the antitumour activity of meso-zeaxanthin and enumerate the mechanism of action.Methods: In vitro cytotoxicity was determined using transformed cells such as Dalton’s lymphoma ascites tumour cel...