An in vitro analysis to ascertain whether smokeless tobacco has any detrimental effect on the diagnostic efficacy of salivary lactate dehydrogenase
Journal Title: International Journal of Medical Science and Public Health - Year 2017, Vol 6, Issue 3
Abstract
Background: Salivary diagnostics promise an easily accessible, non-invasive approach for analyzing and monitoring various oral diseases including cancer. Objectives: This study was undertaken to establish the baseline salivary lactate dehydrogenase (LDH) levels in healthy controls and to discern the effect of smokeless tobacco (SLT) on the diagnostic efficacy of salivary LDH. Materials and Methods: Thirty-seven systemically and periodontally healthy individuals in the age group of 19-27 years without any deleterious habit and oral lesion participated in this study. After explaining the procedure to the participants, unstimulated whole saliva was collected by spit method under sterile conditions. Baseline LDH activity was measured spectrophotometrically. After evaluating baseline activity, 1 ml of saliva was mixed with 1 g of SLT and incubated at 37°C. LDH activity was measured at different time intervals. Results: The median value of baseline LDH levels at rest was 338 IU/L. The gender-based variation in LDH activity was found to be non-significant. Exposure of whole saliva to SLT resulted in an initial increase followed by reduction in the LDH activity. Conclusion: This study provides additional rationale for the use of salivary LDH as a biomarker to investigate the aggressiveness and biological nature of oral pathological conditions. Interpretation of changes in salivary LDH activity should be done with caution in individuals habituated to tobacco use.
Authors and Affiliations
Tajindra Singh Saluja, Anita Spadigam, Shaheen Syed
Keywords
Related Articles
- EP ID EP308569
- DOI 10.5455/ijmsph.2017.0955113102016
- Views 78
- Downloads 0
Effect of modular-based community learning about diabetes mellitus among undergraduate students
Background: Community learning with the help of academic module is an active learning experience for medical students to learn by observation, develop communication skill, participatory action research, and confidenc...
Correlates of contraceptive use among married women of reproductive age group in Adilabad District
Background: India was the first country in the world to launch a family planning program in 1952 with the objective of reducing birth rate to the extent necessary to stabilize the population. Inspite of availability of a...
Purple urine bag syndrome: a case report
Purple urine bag syndrome (PUBS) is a unique disease entity characterized by an alarming purple discoloration of the urine secondary to bacterial urinary tract infection with indigo- and indirubin-producing bacteria. T...
Prevalence and risk factors of Internet addiction in medical students
Background: Technology has a high influence on our lifestyle. Due to this technology, the virtual life of Internet has taken away the real-life experiences. This has significant bearing on both the physical and mental he...
Perioperative transfusions adversely affect post-operative outcomes in less than 18-year undergoing surgical repairs on cardiopulmonary bypass
Background: Cardiopulmonary bypass (CPB) induced hemodilution and coagulopathies are important causes of perioperative bleeding following heart surgeries results both in pediatric and adult patients. However, this is mor...