An Investigation on Measurement Means of Citrinin Toxin Quantity by Toxigenic Aspergillus Species in Biomass, Using ELISA
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2014, Vol 2, Issue 8
Abstract
Objective: Citrinin is a mycotoxin produced by several species of the genera Aspergillus and Penicillium and occurs mainly in food products and animal feed. Some scientific reports show a link between Citrinin and nephrotoxic and possibly a carcinogenic effect for humans. Methods: Samples collected by settling plates,in northern Iran and pure culture isolation performed till the toxin measurement to be done in cell extracts (biomass) which prepared by merging culture in separated prepared culture media incubation. The amount of toxin measured by extracting solutions (50% Acetone, 25% Alcohol Methylic,25% Alcohol Ethylic and PBS washing proposed) using Direct Competitive ELISA. Results: In conducted research, the relative distribution per obtained Aspergillus species isolates for biomass preparation showed that the most frequent were A.ostianus, A.fumigatus,A.niveus,A.niger,A.awamori and A.parasiticus respectively with a prevalence (28.6%), according to averaged Citrinin, indicated that the potent species was A.niger (2009.3ppb) whereas the lowest observed by A.wentii (18.46 ppb).According to maximum Citrinin limits (200ppb) in food products and animal feed including especially related to the Aspergilli and Penicilli, as the result of our measurements and the performed statistical analysis , the maximum amount of Citrinin in the cell extracts, respectively were determined (2009.3ppb) and always produced by A.niger , that were too much more than the universal and local standards allowance enable us to introduce it for all biotechnologic or toxicologic research mentions eventhought as a scientific opinion on the risks for public and animal health related to the presence of Citrinin in food and feed at the targeted geographic area.
Authors and Affiliations
Pouya Nejati| Division Microbiology,Department of Molecular and Cell biology, Faculty of Basic Sciences, Lahijan Branch,Islamic Azad University(IAU), Lahijan, Iran, Arash Chaychi Nosrati| Division Microbiology,Department of Molecular and Cell biology, Faculty of Basic Sciences, Lahijan Branch,Islamic Azad University(IAU), Lahijan, Iran, Mansour Bayat| Division Mycology¶sitology, Department of Pathobiology, Faculty of Veterinary Medical Sciences, Science & Research Branch, Islamic Azad University (IAU),Tehran, Iran, omid lakzaie azar| Division Microbiology,Department of Molecular and Cell biology, Faculty of Basic Sciences, Lahijan Branch,Islamic Azad University(IAU), Lahijan, Iran
Keywords
Related Articles
- EP ID EP13341
- DOI -
- Views 302
- Downloads 14
Prediction of Antibiotics Residues in Raw Milk by Using Binary Logistic Regression Model
medical compounds, especially antibiotics, in which remains in milk and dairy products on the one hand causes health problems such as allergic reactions and Development of bacterial resistance to antibiotics are a seri...
Assessment of Desertification Risk in agricultural land in south of Iran
In many countries, studies to determine the severity of desertification and providing land plans to implement desertification. One of the models is modified MEDALUS or Iranian model (IMDPA), has been used in present re...
Effects of olive leaf on blood metabolites and humoral immunity response of broiler chickens
This experiment was conducted to investigate the effects of olive leaf (OL) on immune response and some blood metabolites of broiler chickens. A total of 400 one-day-old broiler chicks (male and female) were randomly a...
Analysis Students’ Motivation in Vocational Schools and Agricultural Training Centers in Kermanshah Province toward Studying the Field of Agriculture
Objective: Today people are aware of the important role of agriculture as a vital source in their life. Development in agriculture and making healthy food for human, needs trained man. Vocational schools and agricultur...
Effect of Borage Powder on Blood Cells and Immune System
This study was conducted to compare the effects of diets Borage Powder and control (no additives diets) on broiler’s average blood cell counts and immune system on the broiler chicks. Sixty four male broiler chicks (Ro...