Analgesic, pro and anti-inflammatory activities of Desplatsia dewevrei; Cytokine gene expression using Wistar rats and mice
Journal Title: UNKNOWN - Year 2018, Vol 7, Issue 2
Abstract
Objective: Painkilling potential of Desplatsia dewevrei methanol leaf extract was examined using the acetic acid-induced writhing and hot-plate tests. Materials & Methods: Acute anti-inflammatory effect was studied using xylene-induced ear edema and carrageenan induced paw edema models. Gene expression using RT-PCR method was used to query TNF-α, resistin and adiponectin in Wistar rats after a 3-day administration of Desplatsia dewevrei. Results: Desplatsia dewevrei extract significantly (p<0.05) decreased the number of writhes in mice at 30 mg/kg when compared to the control and Aspirin. In the hot plate induced pain test, 10 mg/kg of extract triggered comparable analgesic effect as morphine up to 2 hrs after drug administration. There was significant decrease (p<0.05) in xylene-induced ear oedema at 10 and 30 mg/kg doses of the extract with 80% and 30% when compared to control and dexamethasone. For Carrageenan-induced paw oedema in rats, 30 mg/kg elicited equal effect as indomethacin at 10 mg/kg respectively. The downward regulation/expression of TNF-α, resistin and adiponectin in contrast with control and the expression of beta-actin further indicates that Desplatsia dewevrei has both pro and anti-inflammatory activities. Conclusion: Desplatsia dewevrei methanol leaf extract is anti-inflammatory and elicits both peripherally and centrally analgesic effect.
Authors and Affiliations
Oghale Ovuakporie-Uvo, MacDonald Idu, Philip Obarisiagbon, Callistus Abode
Ethnopharmacological Study of Herbal Medicines used to treat Cancer in Morocco
Since the dawn of time, humans use plants to relieve their ills and diseases; cancer is no exception to this rule. Traditional medicine is an important part of health care but often underestimated. Aim of the study: The...
Cytotoxicological evaluation of semi-purified extracts of some dye yielding plants of the Kashmir Valley on Normal Intestinal Cell Line (IEC-6) by MTT assay
Plant extracts are widely used in many fields and there is a need to evaluate their cytotoxic effect to determine their non-cytotoxic concentration at which they can be used in a safe manner. Keeping this in view, the pr...
Pharmacological activity of Fumaria indica - A review
Fumaria indica (Hausskn.) Pugsley (Fumariaceae), known as “Fumitory”, is an annual herb found as a common weed all over the plains of India and Pakistan. Plant is used widely used in Unani and Ayurvedic system of medicin...
Thermoxidized palm oil diet (TPO)-induced haematological derangements in rats is ameliorated by Aloe vera and Garlic
Objective(s): Therapeutic effects of garlic/Aloe vera on TPO-induced derangements in some haematological parameters and cytoarchitecture of the bone marrow in rats were studied. Materials and Methods: 30 male wistar rats...
Analgesic, pro and anti-inflammatory activities of Desplatsia dewevrei; Cytokine gene expression using Wistar rats and mice
Objective: Painkilling potential of Desplatsia dewevrei methanol leaf extract was examined using the acetic acid-induced writhing and hot-plate tests. Materials & Methods: Acute anti-inflammatory effect was studied using...