Analysis of aflatoxin B1 and aflatoxigenic mold in commercial poultry feeds in Tamil Nadu, India
Journal Title: Journal of Applied and Natural Science - Year 2017, Vol 9, Issue 1
Abstract
A total of 48 commercial poultry feed samples collected from different poultry feed manufactures in Tamil Nadu, India were examined for the contamination of aflatoxin B1 (AFB1) and Aspergillus flavus. AFB1 in the samples was estimated by sandwich ELISA and the presence of A. flavus was detected by Real-Time PCR assay. Real-Time PCR analysis using A. flavus- specific omt primers confirmed the presence of A. flavus in all the samples tested. ELISA results indicated that the AFB1 contents in the poultry feeds ranged from 1.0 to18.7 ppb, which were below the permissible safe limits for poultry bird consumption and health. The results suggest adoption of good man-ufacturing practices by the commercial poultry feed manufacturers during procurement of feed ingredients, handling, storage and processing which might have suppressed the growth of A. flavus and aflatoxin contamination.
Authors and Affiliations
Y. Sireesha, J. Vimal Keerthana, G. Sarathchandra, R. Velazhahan
Synergetic effect of onion (Allium cepa), tomato (Solanum lycopersicum) and garlic (Allium sativum) on in vitro iron bioaccessibility from cooked dehusked mungbean
The usage of combination of onion (Allium cepa), tomato (Solanum lycopersicum) and garlic (Allium sativum) in legume preparations is vogue in North India but the amounts of these three additives need to be optimized to e...
Effect of different sources of potassium on yield and quality of apple (cv. Red Delicious) in temperate conditions
The present investigation was carried out to observe the effect of potassium on different parameters of apple in temperate conditions. Randomized block design experiment was adapted for an orchard of 15 years old with co...
Effect of three decade long application of chemical fertilizer and amendments on crop yield under maize - wheat cropping system in an acid alfisol
The results on the effect of three decade long term application of chemical fertilizers and amendments on the yield of continuous maize-wheat crop rotation in an acid alfisol at Palampur revealed that continuous omission...
Effect of water dipping on separation techniques of pomegranate (Punica granatum L.) arils
For easy separation of arils, pomegranate fruits were subjected to hot water dipping and normal water dipping treatments. Minimum time of separation as 4.10 min/kg of fruit was observed in case of hot water (80±2°C) dip...
Inhibitory effect of bacterial antagonists on the growth of Macrophomina phaseolina (Tassi.) Goid. Causing charcoal rot of sunflower (Helianthus annus L.) in vitro
Charcoal rot caused by Macrophominaphaseolinais a major disease causing in sunflower plant. The pathogen invasion occurs from the seedling to maturity stage. To overcome this problem in vitro, sensitivity of M. phaseolin...