Analysis of Antibiotic Susceptibility Profile and RAPD Typing of Listeria Monocytogenes Isolates
Journal Title: Journal of Health Sciences and Technology - Year 2017, Vol 1, Issue 1
Abstract
Background: The aim of the present study was to analyze antibiotic susceptibility profileand genetic diversity of L. monocytogenes, isolated from chicken carcasses using RAPD-PCR. Methods: In this study, 26 isolates of Listeria monocytogenes recovered from chicken carcasses were used. Antibiotic susceptibility of these isolates were examined for eleven antimicrobial agents and RAPD-PCR with use of three different primers were applied to detect genetic diversity of the isolates. Results: Some of the isolates showed multidrug resistance to commonly used antibiotics. Totally, 26 L. monocytogenes isolates had 16 different antibiogram patterns. Isolates of A and B clusters showed 7 and 6 different pattern, respectively. 12 Isolates of cluster C showed 7 different pattern. Conclusions: Some of isolates had multidrug resistance to antibiotics. This may raise a public health hazard. RAPD-PCR showed that L. monocytogenes isolated from chicken carcasses had low genetic diversity and there was no clonalrelationship between the isolates.
Authors and Affiliations
Tayebeh Zeinali, Abdolah Jamshidi, Mehrnaz Rad, Mohammadreza Bassami
Keywords
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