Analysis of Autosomal SNPs in Paraffin-embedded Tissue Samples: Two Cases

Journal Title: Journal of Forensic Investigation - Year 2015, Vol 3, Issue 1

Abstract

Despite the numerous STR loci available for analysis of cases submitted to forensic laboratories, namely with the use of commercial kits that amplify mini - short tandem repeats (STRs), some cases remain “unsolved” i.e. fail to yield a STR DNA profiling result or result in likelihood ratio calculations that are not sufficiently high. Autosomal single nucleotide polymorphisms (SNPs) may constitute a potential advantage to solve some of these difficult cases, as has already been described by others. In this work we present two revisited cases of our routine work, where the type of samples available for analysis was histological blocks of paraffin-embedded tissue samples from a man (Case 1-“lymph node”) and from a woman (Case 2). The application of autosomal SNPs in the analysis of paraffin-embedded tissues constitutes one of the possible approaches that can be used in our laboratory in some “difficult” cases, and is part of a validation process of the described methodology. Following extraction the two sample DNAs had already been genotyped with the routine commercial STR or mini-STR kits, like AmpFLSTR® Identifiler®, MiniFilerTM and NGMTM PCR Amplification Kits and compared to their respective reference samples. These samples were then typed for the 52 loci included in the in the SNP for ID 52 plex using a SNaPshotTM assay previously described by Sanchez et al. in order to test and validate this multiplex in real casework [1]. The study showed that autosomal SNP can be used as additional markers to the profiling of difficult samples such as paraffin embedded tissue.

Authors and Affiliations

M Lurdes Pontes

Keywords

Related Articles

DNA Microarray Analysis of Hypothermic Murine Myocardium to Study Pathophysiology and Identify Forensic Biomarkers

We used DNA microarray technology to analyze the myocardial transcriptome of mice killed via experimentally induced hypothermia. This analysis identified significant differential regulation of 3438 genes; specifically, 1...

A Review of Methodology for Testing Hair for Cocaine

Cocaine is one of the most commonly abused drugs in the world due to its anesthetic and stimulating effects. Although hair testing is not suitable for the detection of a single usage of cocaine such as in roadside testin...

Unobtrusive and Highly Accurate Breath Alcohol Analysis Enabled by Improved Methodology and Technology

The study objective was to evaluate a novel method and technology for unobtrusive determination of breath alcohol in relation to current industrial accuracy standards. The methodology uses carbon dioxide as a tracer gas...

Complex Variability within the THCA and CBDA Synthase Genes in Cannabis SpeciesTM Identity Panel

The genes encoding the enzymes THCA and CBDA synthase, enzymes catalyzing the final biosynthetic steps in the production of the cannabinoids Δ-9-tetrahydrocannabinolic acid (THCA) and cannabidiolic acid (CBDA), have been...

Evaluating the Use of Differential Scanning Calorimetry for the Analysis of Illicit Substances and Their Adulterants

Chemical profiling of seized material is a powerful tool used by law enforcement agencies to determine the presence of illicit drugs and their adulterants within a suspect material. This study presents a proof-of-concept...

Download PDF file
  • EP ID EP202502
  • DOI 10.13188/2330-0396.1000019
  • Views 129
  • Downloads 0

How To Cite

M Lurdes Pontes (2015). Analysis of Autosomal SNPs in Paraffin-embedded Tissue Samples: Two Cases. Journal of Forensic Investigation, 3(1), 1-4. https://europub.co.uk/articles/-A-202502