ANALYSIS OF CYTOKINES LEVELS IN PATIENTS HAVING HAV DEPENDING ON GENOTYPE AND A PERIOD OF DISEASE DURATION

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ANALYSIS OF CYTOKINES LEVELS IN PATIENTS HAVING HAV DEPENDING ON GENOTYPE AND A PERIOD OF DISEASE DURATION

Journal Title: Annals of Mechnikov Institute - Year 2019, Vol 0, Issue 1

Abstract

Introduction. This article is devoted to the research of connection between different viral Hepatitis A genotypes revealed in Ukrainian territory and peculiarities of the main cytokines production.(INF а IL-2 IL -4). Matherial & methods. During the dissertation 141 patients having HAV were examined. The medical check-up was conducting in the next dynamic:on the inflammation stage and in the early recovering period. Molecular-genetic investigations were carried out with PCR method. Genotyping was conducting with the help of restrictive fragment length polymorphisms analysis method. In IL 2 IL 4 ФНА а researching were applied such testing systems as ООО Pretein contur (Russia St.-Petersburg).Analysis of statistics and processing results were made with the help of the following program Statistika for Windows(Stat Soft Inc USA). Results & discussion. During cytokines levels research on the inflammation stage of disease depending on HAV genotype was revealed that the patients with 3 A genotype were marked with significant increase in anti-inflammatory TNF а concentration by 1.6(p 0,001) times and regulating IL 2 by 3.1 (p 0,001) times and decrease anti-inflammatory IL -4 by 2,3 (0,001) times as well in comparison with patients having 1 A genotype. When analysing rank structure of cytokines deviation degree in groups with different genotypes HAV was found out that the first rank in 1 А TNF group possesses increase of IL 2 level and the last rank in this category has increase of TNF а. It’s exactly the opposite situation for patients with genotype 3 A HAV –have increase of TNF а level on the dominate rank position and IL -2 belongs to the last rank category. Total results indicate on the relations between HAV genotype and functional specification of cytokines link. In this case according to the table 1,the potentional difference between 1 A and 3 A lies in IL -4 production. Taking into account that IL -4 is produced by Tx2 Lymphocytes and IL -2 by Tx1 Lymphocytes there was undertaken the analysis in order to elect the character of Tx1/Tx2 balance in the ratio of IL -2 / IL -4 indexes. It implies that the state of cytokines exchanges that patients with genotype 3 A have shows us the prevailing of Tx2 Lymphocytes response and developing of cell-mediated immune protection defect. Comparisons between cytokines indexes in patients with different genotypes ВГА during the early recovering stage revealed that patients with 3 A genotype in comparison with ones having 1 A genotype own much higher level of Іл-4 amounts (p 0,005) increased thus by 1.7 times and inaccurate growing of TNF а (p 0,05) and decrease of IL -2 (p 0,05). When analysing rank structure of cytokines deviation degree in groups with different genotypes HAV on the early recovering stage it was revealed that their difference lies in rank positions IL -2 and IL -4. If 1 A genotype patients have significant increase of IL -2 amounts and thus it takes the first rank, then decrease of Іл-4 amounts takes the second rank. According to 3 A genotype patients-they have increase of IL -4 amounts on the first rank position and increase of IL -2 takes the second position in the rank position. This represents the existence of specific metabolism of cytokines and ВГА genotype. Prevailing of Tx2 Lymphocytes in 3 A genotype patients unfolds probably the developing of cell-mediated immune protection defect and it also likely acts the role of the unfavourable ВГА duration predictor. Conclusion. Learning cytokines status in ВГА patients has revealed the essential differences depending on the genotype. Thereby 3 A genotype patients in comparison with 1 A genotype ones have more emphasized (p 0,001) cytokines level deviation from controlling indicators on the inflammation stage. The main features of CIC homeostasis disbalance is in the following things: 1 A genotype patients have predominance of IL-2 over IL -4 and Contrariwise 3 A genotype patients have predominance of IL -4 over IL -2.This fact gives evidence of the developing of cell-mediated immune protection defect. 3 A genotype patients in comparison with 1 A genotype patients are identified as having rapid growth of normal amounts of cytokines in blood serum and this leads to the protracted disease stage.

Authors and Affiliations

V. P. , Maliy, I. N. , Asoyan, V. V. Bojko

Keywords

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EP ID EP613618
DOI 10.5281/zenodo.2639486
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