Analysis of Gene Expression in a Gut Epithelial Cell Line Treated with re:iimmune and Lipopolysaccharide
Journal Title: Journal of Nutrition & Health - Year 2016, Vol 2, Issue 2
Abstract
Background: Re:iimmune is a novel oral hydration and probiotic supplement designed to provide clinical strength hydration and intestinal immune support following illness and hospitalization. The goal of this study was to evaluate any anti-inflammatory properties of the supplement due to the inclusion of ginger extract and zinc oxide in the product formulation. Methods: A gut epithelial cell line was stimulated using lipopolysaccharide (LPS) before the addition of the re:iimmune product to the culture medium. Following incubation, gene expression arrays were used to assay 92 different markers involved in a wide-variety of inflammatory and anti-inflammatory processes. Additionally, ten hallmark pro-inflammatory proteins were assayed using immunofluorescent imaging. Results: Findings from qPCR assays indicate that five inflammatory markers were significantly altered as a result of incubation with the re:iimmune product, including the two genes involved in the synthesis and binding of prostaglandins. Immunofluorescent techniques revealed that the re:iimmune product was capable of attenuating LPS-induced inflammation in hallmark pro-inflammatory proteins, including TNF-α and NF-κB. Conclusions: While the mechanism remains unclear, the results from this study indicate that the inclusion of zinc and ginger extract in the formulation of the re:iimmune product may serve to reduce cellular inflammation through the attenuation of pro-inflammatory cytokines.
Authors and Affiliations
Rhy Norton
Keywords
Related Articles
- EP ID EP206006
- DOI 10.13188/2469-4185.1000021
- Views 93
- Downloads 0
Thyroid Function and Phytoestrogens in General US Population
Data from National Health and Nutrition Examination Survey for the years 2007-2010 were used to evaluate the association between serum levels of six thyroid variables, namely, thyroid stimulating hormone (TSH), free and...
Relationship Between Lipoprotein(A) Level and Dietary Intake of Trans and Saturated Fatty Acids in a West Java Province, Indonesia Population
In Indonesia, cardiovascular disease (CVD), which can be related to diet, is a major public health problem. Diets high in fat, saturated fatty acids, and trans fatty acids may increase lipoprotein(a) levels. This cross-s...
Relationship between Prevalence of Overweight and Obesity inAdolescent Girls, and Socio-Economic Status of their Families: A case study in Tehran
Background: Obesity of adolescents is a complicated health problem which depends on several factors like obesity of parents, childhood era nutrition, socio-economic condition, and physical activity. Its main goal was to...
The Nutritional Status of Patients with Diffuse Large B cell Lymphoma Does it Matter?
Background: Nutritional status of lymphoma is not routinely assessed. The aim of this study was to assess nutritional status at the beginning of the treatment and to evaluate its value as predictor of progression free su...
Addition of Alpha-Amylase and Thickener to Blenderized Rice Provides Suitable Viscosity for Use in Nutritional Support
Background: Blenderized diets have been widely adopted as nutritional support, particularly for patients receiving formulae via an enteral route. Although the viscosity of thickened formulae is a determinant of adverse e...