Analysis of Haemoglobin Variants and Oxygen Affinity in Indian and Kyrgyz Population Groups
Journal Title: International Journal of Current Research and Review - Year 2017, Vol 9, Issue 24
Abstract
Aim: The hemoglobin (Hb) variants can alter the structure and biochemical functions of Hb along with affecting physiological properties of the individual. In normal adult RBC, Hb variants that exist are HbA1, HbA2, HbS and HbF. Mutations in globin chains can change the Hb-O2 affinity which can alter the normal loading of oxygen in lungs and unloading in the tissues and also affect arterial oxygen saturation. Absence of abnormal Hbs rules out upto some extent presence of genetic or acquired facilitated oxygen affinity. Methodology: The study was undertaken to compare haemoglobin variants using HPLC in Indians (n=10) and Kyrgyz population groups (n=10). We estimated oxygen affinity (P50) from PO2, SO2 and pH values using Lichtman equation and peripheral oxygen saturation (SpO2) using pulse oximetry at basal and upon acute HA exposure to 4111m in both the population groups. Results: We obtained Hb fractions A1a, A1b, A1c and A2 within normal range and no HbF or HbS fractions were found in any volunteer from HPLC run. All the P50 values were well within normal range (22-28mmHg). No change in P50 was observed upon HA exposure in these two populations but Kyrgyz have been found to have a higher P50 compared to Indians at basal. Discussion and Conclusion: Since our data rule-out the existence of abnormal Hb variant in either of the population, thus the significant decrease in SpO2 observed upon HA induction in both the groups is due to hypoxia at HA and higher P50 in Kyrgyz compared to Indians is population specific.
Authors and Affiliations
Praveen Vats
Keywords
Related Articles
- EP ID EP598773
- DOI 10.7324/IJCRR.2017.9247
- Views 119
- Downloads 0
Prevalence and Factors Influencing Depression in Patients with Stroke Attending a Tertiary Care Teaching Hospital, a Cross Sectional Study
Background: Stroke is a major public health problem contributing to significant morbidity and mortality across the globe. In spite of many reviews documenting a strong association between stroke and depression, it is the...
The Configuration and Variations in the Developing Circle of Willis – A Retrospective Autopsy Study
Introduction: Cerebral hemodynamics undergoes a shift from carotid system to vertebro-basilar system from fetal stage to adult life. The circle of Willis (CW) plays a major role in redistributing the blood and enables in...
Significance of Aldose Reductase in Diabetic cataract
Background: Cataract is a major cause of blindness. Diabetes mellitus is a major risk factor for the development of cataract. Diabetic patients have 25 times higher risk of cataract than non-diabetic population. The meta...
Chlorpyrifos Impairs the Contraction of Uterine Smooth Muscle by Inhibiting the Secretion of Estradiol from Ovary in Rat
Objectives: Chlorpyrifos (CPF) has been used heedlessly in India to protect crop plants from pest induced crop loss. The objective of the study was to examine the effect of CPF, a broad spectrum organophosphate pesticide...
Evaluation of Salivary Total Protein in Insulin Dependent Diabetes Mellitus (IDDM) Patients
Aims: To evaluate salivary total protein in IDDM patients and to compare with healthy non-diabetic control group. Methods and Material: The study consisted of 50 IDDM patients and 50 age matched healthy controls. All the...