Analysis of Pralatrexate Using Simple and Fast Reverse Phase High Performance Liquid Chromatographic Method
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 6
Abstract
A simple, specific, precise, accurate, and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the determination of Pralatrexate in both pure and pharmaceutical dosage forms. In this method Agilent (4.6×150mm) 5µ column in isocratic mode with mobile phase containing water: methanol (25:75% v/v) was selected. The effluents were monitored at 300 nm and flow rate was fixed as 1.4 ml / min. The retention time was 3.312 min. The linearity was in the range of 20-100 g / ml. This method was validated for linearity, precision, limit of detection, limit of quantification and accuracy. Statistical analysis proves that the method is precise, reproducible and selective for the estimation of the pralatrexate drug.
Authors and Affiliations
Syeda Kulsum, G Vidya Sagar
Keywords
Related Articles
- EP ID EP387021
- DOI 10.9790/3008-110604116118
- Views 96
- Downloads 0
Betterment the production of prodigiosin that was produced from Serratia marcescen by means mutations
Serratia marcescens was exposed to mutation for becoming better the production of prodigiosin (quantitatively and qualitatively) and examined it as antibacterial activities. One isolate of S.marcescen (Wild) was exposed...
Phytochemical Compositions of Fruits of Three Musa Species at Three Stages of Development
This was designed to evaluates the phytochemical compositions of fruits of three Musa species at three stages of development. Spectrophotometric methods were used for the study. Results of the qualitative phytochemical a...
Development of Nimbolide from Seeds of Azadirachta Indica Extract Assisted by Molecular Docking to Cyclin E, against Cervical Cancer
The recent studies on Nimbolide, a robust cervical anticancer agent was identified for the separation of Nimbolide from Azadirachta indica berry collected from Ranga Reddy- Dist, the seeds were initially isolated, dried...
Cauda Equina Syndrome: A Case Report
Cauda equina syndrome (CES) is a rare syndrome which is characterized by low back pain, unilateral or bilateral sciatica, motor weakness of lower extremities, saddle anesthesia, bowel and bladder dysfunction and occasion...
Review, The (medical) benefits and disadvantage of sea cucumber
A remarkable feature of Holothurians is the catch collagen that forms their body wall. Catch collagen has two states, soft and stiff, that are under neurological control [1]. A study [3] provides evidence that the proces...