Analysis of primary metabolites from Verticillium lecanii and studied its immunological aspects of crude protease against Rapither-AB
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2017, Vol 6, Issue 2
Abstract
Objective- The objective of our study is to examine its primary metabolites (protein content) from dried powder of Verticillium lecanii. In addition, immunological studies were conducted in order to analysing the protease content against Rapither-AB using crude enzyme of Verticillium lecanii in virally infected human whole blood samples. Methods- For these studies, quantifying the protein content from Verticillium lecanii using Nanodrop method. In contrast, crude protease isolated from crude enzyme against Rapither-AB in order to analyse its immunological activity against infected blood samples for estimating its proliferation and total cellular content. In addition, Elisa experiment were performed using protein of Verticillium lecanii as coating antigen and measure its antibody profile of various medicinal plants against this protein antigen. Results-The results of these studies showed production of protein at a very low concentration of dried powder, Verticillium lecaniiand also showed the presence of protease content against crude enzyme of Verticillium lecanii containing Rapither AB and claimed its antimicrobial properties at higher doses with respect to decline in proliferation assay and total cellular content in case of virally infected blood samples. In contrast, few of these medicinal plants are able to produce antibody production against this fungal strain i.e. Verticillium lecanii. Conclusion- Overall these studies indicate its antimicrobial properties of protease extracted from crude enzyme of Verticillium lecanii against Rapither AB containing arteether. Keywords: Verticillium lecanii; protein; protease; Rapither AB; arteether
Authors and Affiliations
Pradnya P Raskar
Keywords
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