Analysis of the genetic stucture of certain types of scaled carp / Аналіз генетичної структури окремих типів українського лускатого коропа
Journal Title: Рибогосподарська наука України - Year 2016, Vol 38, Issue 4
Abstract
Purpose. Study of the genetic structure specificity, intra- and interpopulation genetic variation of the brood stocks of Ukrainian scaled carp from different fish farms of Ukraine using DNA markers (ISSR-PCR). Methodology. A PCR (ISSR-PCR) method with appropriately selected primers has been used to investigate the genetic structure specificity. Findings. The genetic structure analysis of Ukrainian scaled carp has been carried out using three microsatellite DNA loci: (AGC)6G, (AСC)6G, (AGC)6С. In total, 55 amplicons were detected for all primers. Fifteen primers were detected when using (AGC)6G primer, 17 for (AСC)6G primer and 23 for (AGC)6С primer. The molecular weight on electropherograms had maximum values when using (AСC)6G primer (500 n.p. – 3500 n.p.) in Nyvky carp. The detected specific differences between the examined populations of scaled carp depend on their genetic origin. Variations of the detected amplicons are sufficient for separating individual fish or, if works are carried out with a group of broodfish, for selecting parental pairs for increasing genetic diversity. Originality. The use of ISSR-markers allowed detecting the peculiarities of the genetic structure, level of genetic variability of the brood stocks of Ukrainian scaled carp. For the first time, we obtained new data on the specificity of the genetic structure when using PCR techniques, which allow detecting specific mechanisms of the maintenance of the relative stability of the genetic material of Ukrainian scaled carps and allow controlling and preserving the specificity of their genetic structure. Practical value. Proposition of a method for the genetic control of Ukrainian scaled carp stocks based on the application of PCR techniques, which allows performing an analysis of the genetic structure of fish stocks and using the genetic information at early stages of breeding selection process.
Authors and Affiliations
А. Маriutsa, S. Tarasjuk, І. Hrytsyniak
Keywords
Related Articles
- EP ID EP474965
- DOI 10.15407/fsu2016.04.113
- Views 61
- Downloads 0
Description age-3 brood rainbow trout bred in the conditions of the industral fish farm "Sloboda Banyliv" / Характеристика 3-річних плідників райдужної форелі, вирощених в умовах індустріального господарства «Слобода Банилів»
Purpose. To analyze the results of cultivation and give fish-breeding and biological characteristics of age-3 brood rainbow trout grown using industrial technology in the conditions of the trout farm "Sloboda Banyliv."...
Black Sea roach (Vyrezub) Rutilus frisii frisii (Nordmann). Thematic bibliography / Вирезуб Rutilus frisii frisii (Nordmann). Тематична бібліографія
Purpose. To create a thematic bibliographic list of publications on studies of ecological and biological characteristics of the Black Sea roach (vyrezub) Rutilus frisii frisii Nordman, as an endangered cyprinid species o...
Effect of mineral betonit on chemical composition of carp (Cyprinus carpio Linnaeus, 1758) muscles / Вплив мінералу бентоніт на хімічний склад м’язів коропа (Cyprinus carpio Linnaeus, 1758)
Purpose. To study the effect of bentonite mineral added to the basic diet of age-1 carps on the content of lipids and their individual classes as well as on the chemical composition of muscles to establish the efficiency...
USE OF FEED YEAST IN FEEDING OF STURGEON (ACIPENSERINAE) SPECIES (REVIEW) / ВИКОРИСТАННЯ КОРМОВИХ ДРІЖДЖІВ У ГОДІВЛІ ОСЕТРОВИХ (ACIPENSERINAE) ВИДІВ РИБ (ОГЛЯД)
Purpose. To review scientific sources on the use of feed yeast preparations in feeding of sturgeon species (Acipenserinae). Findings. The review of scientific works demonstrated that feed yeast in the feeding of sturgeo...
PECULIARITIES OF RAISING YOUNG-OF-THE-YEAR EUROPEAN GRAYLING (THYMALLUS THYMALLUS L.) / ОСОБЛИВОСТІ ВИРОЩУВАННЯ ЦЬОГОЛІТОК ХАРІУСА ЄВРОПЕЙСЬКОГО (THYMALLUS THYMALLUS L.)
Purpose. The study was aimed at raising young-of-the-year European grayling and improving the industrial technology with the use of specialized artificial feeds. Methodology. The work on raising young-of-the-year Europe...