ANALYSIS OF THREE CARDIOVASCULAR DRUGS IN THEIR TERNARY MIXTURE USING GREEN ANALYTICAL METHODOLOGY OF SMART SPECTROPHOTOMETRIC METHODS AND RP-HPLC METHOD
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 8
Abstract
Objective: Development and validation of two new, simple and specific analytical techniques for the simultaneous determination of a ternary mixture of co-administered cardiovascular drugs ticagrelor (TICA), irbesartan (IRB) and hydrochlorothiazide (HCT).Methods: Chemometric assisted UV spectrophotometric methods (considered as green analytical chemistry) and RP-HPLC method were developed and validated. The different applied chemometric methods are based on Fourier function convolution (FF) and Double divisor ratio spectra Fourier function (FFR). As for the HPLC method, it was developed on a C18 Sunfire© waters column with a mobile phase composed of Acetonitrile: sodium dihydrogen phosphate (adjusted to pH 3.6 using orthophosphoric acid) in the ratio of 60:40 v/v at a flow rate of 1 ml/min. Quantification was based on measuring peak areas at 230 nm.Results: By applying the chemometric assisted methods, good percentage recoveries were obtained in quantifying each of the drugs in their triple mixture. The RP-HPLC method, peaks were well resolved with retention times 4.6, 8.0 and 10.0 min, for HCT, TICA and IRB, respectively. The established method was also applied in samples spiked with plasma; the peaks were well separated from each other and from plasma protein peaks, proving the ability of the HPLC method to be applied in biological samples. The methods (RP-HPLC and chemometric assisted methods) were validated in terms of linearity, LOD, LOQ, precision and accuracy. The results obtained from the analysis of the co-administered mixture by the proposed chemometric assisted methods were statistically compared to those obtained by the applied RP-HPLC method.Conclusion: The applied RP-HPLC methods were proved to be more sensitive when compared to the applied chemometric methods, thus could be used for determination of drug concentration in human plasma. However, the applied chemometric methods, considered as green analytical chemistry, is a simple, time-saving method that requires minimal use of a hazardous solvent.Â
Authors and Affiliations
Marwa K. El Jamal, Azza A. Gazy
Keywords
Related Articles
- EP ID EP576139
- DOI -
- Views 61
- Downloads 0
ETIOLOGICAL FACTORS FOR THE DEVELOPMENT OF LUNG CANCER IN NON-SMOKERS: AN OVERVIEW
Lung cancer is the most frequent malignant tumour with the highest mortality rate around the world, leading to greater than a million deaths annually. The number of deaths due to lung cancer is expected to increase to te...
ANTIOXIDANT, ANTIMICROBIAL, ANTIPROLIFERATIVE ACTIVITIES AND IN SILICO DRUG LIKENESS PREDICTION OF PURIFIED MHH COMPOUND, ISOLATED FROM MILLINGTONIA HORTENSIS LINN
Objective: To investigate the antioxidant, antimicrobial, antiproliferative activities and in silico drug likeness prediction of the MHH compound.Methods: The antioxidant, antimicrobial and antiproliferative activities o...
EVALUATION THE EFFECT OF NANOTECHNOLOGY ON PHARMACEUTICAL AND BIOLOGICAL PROPERTIES OF METRONIDAZOLE
This article has been withdrawn from publication on author’srequest
COMPARATIVE STUDIES OF EFFICACY AND EFFECTS ON OXIDATIVE STRESS OF AMLODIPINE AND RAMIPRIL IN THE HYPERTENSIVE PATIENTS OF NORTH EAST INDIA
Objective: To compare the efficacy and the effect of the oxidative stress of Calcium Channel Blocker (CCB), Amlodipine and Angiotensin Converting Enzyme (ACE) inhibitor, Ramipril in the hypertensive patients of North Eas...
DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHOD FOR THE ESTIMATION OF LINEZOLID IN LINEZOLID GEL
Objective: Development and validation of new RP-HPLC method for the estimation of linezolid in linezolid gel.Methods: Linezolid was chromatographed on a reverse phase symmetry C18 column (150 x 4.6 mm x 3.5 µm) in a mob...