Analysis on phenotype and genotyping of CisAB subtypes: 12 Cases
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 12
Abstract
Objective To analyze and master the serological and genetic characteristics of the samples with CisAB subtype and their genetic background. Methods From January 2018 to January 2022, blood samples with discrepant ABO blood typing results, from Zhengzhou voluntary blood donors and hospital patients, were subjected to phenotypic classification using micro column gel card and tube method, as well as amplification of exons 6 and 7 in ABO gene using PCR. The pedigrees of individuals with the same CisAB subtype but different serological typing results in the same family were analyzed. Results 11 The forward typing of 12 samples was AB type, and unexpected antibodies against weaker antigens were found in 11 serum samples, including 9 cases with strong antigen A, 2 cases with strong antigen B, and 1 case with consistent forward and reverse typing results. Gene sequencing confirmed that 11 cases were CisAB01 subtype and 1 case was CisAB05 subtype.Among them, 7 cases had the genotype of CisAB01/O and serological phenotype of A2B3; 2 cases had the genotype of CisAB01/B and phenotype of A2B; 2 cases had the genotype of CisAB01/A and serological phenotype of A1Bx and A1B3; 1 case had the phenotype of AxB. In the CisAB01 family, 1 case of CisAB01/O with A2B3 phenotype and 1 case of CisAB01/B with A2B phenotype were detected. In the CisAB05 family, 2 CisAB05/O01 and 1 CisAB05/O02 were detected. Conclusion The serological phenotypes of different individuals in the same CisAB01 family can be different when paired with different ABO alleles. It is advisable to accurately identify the CisAB subtype genes with molecular biological methods to ensure blood transfusion safety.
Authors and Affiliations
Xiaoli MA, Minglu GENG, Jianbin LI, Hecai YANG, Liping WANG, Yi CAO, Jiaxuan LIU
Keywords
Related Articles
- EP ID EP738427
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.12.004
- Views 55
- Downloads 0
HIV antiretroviral therapy among blood donors: the impact on blood safety
Objective To explore the the potential risks of antiretroviral therapy(ART) drugs on blood safety among blood donors in Shenzhen. Methods High pressure liquid chromatography coupled with tandem mass spectrometry (HPLC-MS...
Discussion on the collection and supply mode of RhD negative blood suitable for prefecture-level city: a retrospective analysis
Objective To explore a RhD negative blood collection and supply mode suitable for Liaocheng area and improve the blood demand satisfaction rate of RhD negative patients. Methods Under different collection and supply mode...
Effects of acute normovolemic hemodilution combined with intraoperative recycled autotransfusion on blood transfusion volume and coagulation function in elderly patients undergoing cardiac surgery
Objective To explore the effects of acute normovolemic hemodilution (ANH) combined with intraoperative recycled autotransfusion on blood transfusion volume and coagulation function in elderly patients undergoing cardiac...
Establishment of internal quality control methodology for blood transfusion compatibility testing
Objective To monitor the effectiveness and accuracy of the blood transfusion compatibility test system by self-made weakly positive internal quality control products. Methods Red blood cells from DAT(-) healthy subjects...
The present situation and advance of red blood cell transfusion triggers
In clinical practice, red blood cell infusion needs to be based on the patient′s hemoglobin level. However, different guidelines recommend different thresholds for red blood cell infusion and the timing of blood transfus...