Analysis on the difference of circulating threshold of polymerase chain reaction in blood screening of external quality assessment
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 9
Abstract
Objective To analyze the difference of circulating threshold (Ct) of polymerase chain reaction (PCR) in blood station laboratories during the external quality assessment, and to put forward suggestions for the quality improvement of participating laboratories. Methods From 2018 to 2021, the blood station laboratories participated in the external laboratory quality assessment of CITIC including blood screening items with nucleic acid testing method. The data of Roche diagnostic reagent group were used as the source, and the detected Ct values of three groups of quality control samples of HBV A subtype (400 IU/mL), HCV 1b subtype (400 IU/mL) and HIV B genotype (500 IU/mL) were used as the objects. The data were grouped according to quality control (sample) batches, reagent batches and different laboratories. Using the statistical method of variance analysis (assuming P<0.05 as significant), the detected Ct value of each group was analyzed. Results For the three items (HBV/HCV/HIV), the grouping data involving 42 batches of quality control (13/12/17), 28 batches of reagent (11/8/9) and 57 laboratories (19/19/19) were selected. The grouping analysis of quality assessment batches shows that there was no significant difference between HBV and HCV quality assessment batches, and there was no significant difference between other HIV batches except the two batches of HIV quality assessment samples released in 2021. The grouping analysis of each reagent batch showed that there was no significant difference between each reagent batch for HCV and HIV detection, while there was significant difference between two batches of HBV reagents. After excluding the data groups with significant differences in the quality control batch groups and the reagent batch groups, the detected Ct value of each laboratory group had extremely significant differences in the three items of HBV, HCV and HIV. Through pairing analysis, it was found that four laboratories had significant differences with most other laboratories in the three items, mainly manifested in the high mean value of Ct. Conclusion For the blood station laboratories with correct test results of quality assessment samples, there are differences in Ct values detected by PCR, which may be mainly caused by the detection ability of the participating laboratories.
Authors and Affiliations
Lidong LIU, Xiaofei WU, Yao JIA, Yuwen HUANG, Qin MO, Hong LIU, Rongna Ma, Zhong XU, Xun WANG
Keywords
Related Articles
- EP ID EP738346
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.09.012
- Views 61
- Downloads 0
Effect and mechanism of extracorporeal photopheresis on preventing acute graft versus host disease in ice
Objective To investigate the preventive effects of early apoptotic splenic mononuclear cells induced by extracorporeal photopheresis (ECP) on acute graft versus host disease (aGVHD) in mice and explore the underlying mec...
Enlightenment from the revision of Standard for Blood Storage
The recently officially approved and released health industry standard Standard for Blood Storage(WS 399-2023) by the National Health Commission is the latest revised version of Standard for Blood Storage(WS 399-2012), w...
Investigation on current Hepatitis E virus infection among voluntary blood donors
Objective To investigate the situation of hepatitis E virus(HEV) infection among voluntary blood donors in Wuhan area and provide evidences for enhancing blood screening strategies. Methods HEV nucleic acid detection(NAT...
Status and serological features of occult hepatitis B virus infection among blood donors in Xuzhou
[Objective] To explore the current status, influencing factors, and serological characteristics of occult hepatitis B virus infection (OBI) among blood donors in Xuzhou, so as to provide data support for improving blood...
Progress in clinical application of autologous ozonized blood transfusion
Autologous ozonized blood transfusion (AOBT) is a therapeutic method that mixes blood with medical ozone in vitro and then reinfuses it into the body. It has been widely used in the auxiliary treatment of ischemia, hypox...