ANALYSIS REACTIVITY OF PUNICA GRANATUM POLYPHENOLS TO THE OSTEOCALCIN, BONE MORPHOGENETIC PROTEIN-2, AND COLLAGEN TYPE-1
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objective: Punica granatum (PG) contains anthocyanins which are useful as antioxidants, anti-inflammatory, and prevent cancer, while also increasing bone cell proliferation and osteoblasts differentiation in bone remodeling.Methods: The reactivity of osteocalcin protein markers with PG polyphenol fractionation hydrogels observed using enzyme-linked immunosorbent assay, the degree of reactivity was determined by optical density at 550 nm.Results: PG butanol fraction has better reactivity compared to the total extract, ethyl, and hexane fraction. Based on the reactivity distribution, bone morphogenetic protein (BMP)-2 and collagen Type-1 had a dominant distribution compared to osteocalcin, but the theses proteins had a strong relation (r = 0.8) with probability (P < 0.05).Conclusion: PG butanol fraction had better reactivity to osteocalcin, BMP-2, and collagen Type-1 compared with total extract, hexane, and ethyl fraction. The four PG polyphenol fractionations have dominant reactivity to BMP-2.
Authors and Affiliations
Edrizal Burhan, Bergman Thahar, Trimurni Abidin, Deddi Prima Putra, Basri A Gani
Keywords
Related Articles
- EP ID EP605269
- DOI 10.22159/ajpcr.2018.v11i12.29683
- Views 120
- Downloads 0
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