ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF FLUPIRITINE MALEATE AND PARACETAMOL BY USING RP-HPLC
Journal Title: Indo American Journal of Pharmaceutical Sciences - Year 2018, Vol 5, Issue 4
Abstract
A new RP-HPLC method for the quantitative determination of Flupiritine Maleate and Paracetamol was developed and validated as per ICH guidelines. The drugs were injected into Azilent HPLC system with SPD-20A Detector and C18 column (250×4.6, 5 µm) maintained at ambient temperature and effluent monitored at 240 nm. The mobile phase consisted of 0.1% Orthophosphoric acid: Methanol (50:50 V/V). The flow rate was maintained at 1.0 ml/min. The calibration curve for flupiritine maleate and paracetamol was linear from 6-300.03µg/ml and 1.25 - 60.08µg/ml (r 2 for Flupiritine Maleate =1.0 and r 2 for Paracetamol = 0.99). The proposed method was adequate, sensitive, reproducible, accurate and precise for the determination of Flupiritine Maleate and Paracetamol in bulk and pharmaceutical dosage form. Keywords: Flupiritine Maleate and Paracetamol, Linearity, Validation.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP283709
- DOI -
- Views 249
- Downloads 0
DESCRIPTIVE STUDY KNOWING THE PATIENTS LOAD IN THE NEONATAL ICU AT THE TERTIARY CARE HOSPITAL LAHORE
Objective: To Know the Number of patients referred to tertiary hospital neonatal intensive care unit (NICU) in Lahore, disease model and outcome determination. Study Design: A descriptive study Location and duration: The...
A VALIDATED STABILITY-INDICATING AND LC-MS COMPATIBLE METHOD FOR THE DETERMINATION OF RELATED SUBSTANCES AND ASSAY OF MONTELUKAST SODIUM AND LEVOCETIRIZINE HCL BY U-HPLC IN TABLET DOSAGE FORM
A novel gradient reverse-phase ultra-high performance liquid chromatography (U-HPLC) method has been developed and validated for determination of related substances and assay of Montelukast sodium and Levocetirizine HCl...
A PROSPECTIVE RESEARCH ON THE FASCIO-CUTANEOUS FLAPS ROLE AS AN INTERVENTION OF THE COVERAGE OF SOFT TISSUE IN (TYPE 111 – B) OPEN TIBIAL FRACTURES
Object: The determination of the fascio-cutaneous flap role as the coverage of the soft tissue in open tibial fractures of type 111 – B as experienced by the professor of Mayo Hospital, Lahore. Material and Methods: Samp...
CORONARY REVASCULARIZATION TECHNIQUES AND THEIR IMPACTS ON CHRONIC KIDNEY DISEASE (CKD) PATIENTS, SERVICES HOSPITAL, LAHORE
Background and Objective: Coronary heart diseases are being treated by surgical and non-surgical procedures like Coronary Artery Bypass Graft (CABG) and Percutaneous Coronary Intervention (PCI). The impact of these techn...
IMPURITY PROFILING AND DRUG CHARACTERIZATION: BACKDROP AND APPROACH
Impurities are always an ‘essential evil’, that will however be present with the drug products with but with the dawn of more safety based limits for controlling the related impure substances (ICH Q3A-D and M7) it can be...