ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NEW STABILITY-INDICATING REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN HYDROCHLORIDE AND EMPAGLIFLOZIN IN TABLET DOSAGE FORM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 1
Abstract
Objective: The objective of this study was to develop and validate a stability-indicating reverse-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of the metformin and empagliflozin in tablet dosage forms.Methods: The chromatographic conditions were optimized and it was run through Std. BDS (250 mm × 4.6 mm, 5 m) column with mobile phase consisting of 0.1% orthophosphoric acid buffer: acetonitrile in the ratio of 50:50. The flow rate was 1 ml/min and optimized wavelength was 210 nm. Temperature was maintained at 30°C.Results: The retention times of metformin and empagliflozin were found to be 2.588 min and 3.679 min and percentage relative standard deviation (RSD) of the metformin and empagliflozin was found to be 0.59 and 1.2, respectively. Percentage recovery was in the range of 100.01–100.65% for metformin and empagliflozin, respectively.Conclusion: A sensitive, rapid, and specific method has been developed for the simultaneous estimation of metformin and empagliflozin using RP-HPLC in tablet dosage form.
Authors and Affiliations
Geetha Susmita A, Rajitha G, Ramya Yadav Y, Uma P
Keywords
Related Articles
- EP ID EP607135
- DOI 10.22159/ajpcr.2019.v12i1.26537
- Views 127
- Downloads 0
DEVELOPMENT AND VALIDATION OF A HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY FOR THE DETERMINATION OF TERBUTALINE SULFATE, BROMHEXINE HYDROCHLORIDE, AND ETOPHYLLINE IN PHARMACEUTICAL DOSAGE FORM
Objective: The study aimed to development and validation of simple, precise, and reliable high-performance thin-layer chromatography (HPTLC) for the determination of terbutaline sulfate (TBS), bromhexine hydrochloride (B...
ANTICANCER AND FREE RADICAL SCAVENGING POTENTIAL OF THE MARINE ALGICOLOUS ENDOPHYTIC FUNGUS CLADOSPORIUM UREDINICOLA
Objective: The objective was to study the antioxidant and anticancer potential of the endophytic fungus Cladosporium uredinicola, isolated from the marine brown alga Dictyota dichotoma. Methods: Anticancer effect of the...
FORMULATION AND EVALUATION OF IN-SITU GEL CONTAINING CIPROFLOXACIN HYDROCHLORIDE IN THE TREATMENT OF PERIODONTITIS.
Objective: The present study describes the use of in-situ gel in periodontal drug delivery systems which contains gellan gum (0.4–0.6% w/v), pluronic F127 (14, 15 and 16% w/v), and drug Ciprofloxacin HCl (0.1% w/v). Nu...
TREATMENT OF VARIOUS DISEASES BY CANNA INDICA L. - A PROMISING HERB
In recent years, ethnobotanical and traditional application of natural compounds, principally of plant origin established much attention as they are well tested for their effectiveness and generally believed to be non-to...
IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA
Objective: Curcuma longa is a known natural medicine for inflammation from ancient times. It has a low absorption rate and poor solubility. Hence, it is used for the green synthesis of nanoparticles. Zinc oxide nanoparti...