Analytical Method Development and Validation of RP-HPLC Method for Estimation of Pazopanib Drug Sample and It’s Dosage Form
Journal Title: International Journal of Experimental Research and Review - Year 2023, Vol 36, Issue 7
Abstract
The study focuses on developing and verifying a cost-effective Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) technique for quantifying Pazopanib HCl in both bulk and tablet forms. The study aims to develop a cost-effective approach for routine quality control analysis by utilizing the simplicity and wide accessibility of HPLC. A Shimadzu C18 column (5 µm, 250 mm × 4.6 mm) was successfully used to separate Pazopanib HCl via chromatography. The mobile phase consisted of a mixture of Potassium dihydrogen phosphate in water (pH 2.9, corrected with Phosphoric Acid) and Acetonitrile in a ratio of 25:75 v/v. The isocratic elution mode was utilized with a flow rate of 1.0 mL/min, a column temperature of 25°C, and an injection volume of 20 µL. Pazopanib hydrochloride had a retention period of 2.8 minutes when measured at an isobestic wavelength of 215 nm. The described RP- HPLC technique showed exceptional specificity, accuracy, precision, linearity, and durability, rendering it a viable instrument for Pazopanib HCl tablets' regular quality control analysis. This method is both efficient in terms of analytical performance and economically profitable, making it very suitable for frequent pharmaceutical analysis.
Authors and Affiliations
Suhas Shivaji Siddheshwar, Akshay Balu Kachare, Someshwar Dattatraya Mankar
Keywords
Related Articles
- EP ID EP726764
- DOI 10.52756/ijerr.2023.v36.019
- Views 78
- Downloads 0
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