ANALYTICAL METHOD DEVELOPMENT, VALIDATION AND FORCED DEGRADATION STUDIES OF LANSOPRAZOLEBY RP-HPLC
Journal Title: Asian Journal Of Pharmaceutical Research and Development - Year 2018, Vol 6, Issue 4
Abstract
The objective of this work is to develop a rapid, precise, accurate and sensitive revere phase liquid chromatographic method and Forced degradation studies for the estimation of Lansoprazole. The chromatographic method was standardized for Lansoprazole using Shimadzu HPLC model reverse phase analytical Inspire grace C18 column (250 mm x 4.5 mm, 5mm particle size) with PC-3000-M Reciprocating Pump (40 Mpa) and UV-3000-M Detector, at 285nm and flow rate of 0.8 ml/min. The mobile phase consists of 80:20 Methanol: water. The linearity of proposed method was investigated in the range of 10-50 μm/ml (R2 = 0.999) of Lansoprazole. The limit of detection (LOD) was found to be 0.12 mm/ml. The limit of quantification (LOQ) was found to be 0.36 mg/ml. The retention time of Lansoprazole found to be 5.4 min. The method was statistically validated and % RSD was found to be less than 2 indicating high degree of accuracy and precision. Hence proposed method can be successfully applied for the estimation of Lansoprazole in further studies
Authors and Affiliations
Madhavi K Meher, Charushila Bhangale
Keywords
Related Articles
- EP ID EP499507
- DOI 10.22270/ajprd.v6i4.387
- Views 105
- Downloads 0
A BRIEF REVIEW ON DENGU
Globally important arboviral infection transmitted by the Aedes genus of mosquito.Endemic in more than 120 countries, particularly the Southeast Asian and Western Pacific regions, the Caribbean, Latin America, and some r...
EBOLA VIRUS: AN OVERVIEW
Ebola virus disease (EVD), also known as Ebola hemorrhagic fever (EHF) or simply Ebola, is a viral hemorrhagic fever of humans and other primates caused by ebola viruses. Signs and symptoms typically start between two da...
PATTERN OF ADVERSE DRUG REACTIONS AND ITS MANAGEMENT IN FEMALE CANCER PATIENTS IN A PRIVATE HOSPITAL IN TELANGANA, INDIA
Chemotherapy and radiation are one of the important components of treatment for many cancers. Anti-neoplastic agents are used with caution due to their high toxicity and narrow therapeutic window. Studies describing patt...
TERPENOIDS AND PHYTOSTEROLS ISOLATED FROM PLUMERIA RUBRA L., FORM ACUTIFOLIA (AIT) WOODSON AND ITS FUNGUS PARASITE COLESPORIUM PLUMERIAE
The hexane and ethyl acetate extracts of the leaves from Plumeria rubra L., form acutifolia (Ait) Woodson and the methanolic extract from the fungus Coleosporium plumeriae Pat. were analized by high resolution gas chroma...
FORMULATION AND EVALUATION OF ORMELOXIFENE FAST DISSOLVING TABLETS
The objectives of the present work were to enhancedissolution and solubility of slightly water soluble ormeloxifene hydrochloride and formulate fast dissolving tablets. The research work was two-phase process; the first...