Analytical Method for the Simultaneous Estimation of Sitagliptin and Simvastatin using RP-HPLC
Journal Title: Journal of Pharmaceutical and Medicinal Chemistry - Year 2019, Vol 5, Issue 1
Abstract
A novel, rapid, precise and accurate high performance liquid chromatographic method was developed and validated for the simultaneous determination of Sitagliptin phosphate and Simvastatin in bulk drug and pharmaceutical formulation. The components were separated on Ymc Cyano (150 mm × 4.6 mm I.D., 5 μm particle size) with a mobile phase composed of 20 mM ammonium formate and acetonitrile in the ratio of 50:50 v/v (Adjust the pH to 3.5 with 0.1% formic acid) at a flow rate of 1.2 mL/min. The response was measured at 218 nm. The peaks were detected at 5.33 minutes and 4.19 minutes for Sitagliptin phosphate and Simvastatin respectively. Calibration curves were found to be linear (r2=0.999 for both Sitagliptin phosphate and Simvastatin respectively) over the concentration range of 2.5-200 µg/mL for Sitagliptin phosphate and 1-80 µg/mL for Simvastatin. The method was validated for linearity, precision, accuracy, ruggedness and robustness. The proposed method can be applicable for simultaneous quantitation of Sitagliptin phosphate and Simvastatin in tablet dosage form. Validation results assured that the recommended method was specific, rapid, reliable and reproducible. Good percent recoveries and low % RSD reveals the suitability of the present method for analysis of Sitagliptin phosphate and Simvastatin in quality control laboratories.
Authors and Affiliations
A. Lakshmana Rao
Keywords
Related Articles
- EP ID EP649489
- DOI 10.21088/jpmc.2395.6615.5119.1
- Views 110
- Downloads 0
Effect of Antibiotics, Rehydration Therapy, Zinc and Probiotics in Diarrhoea in Children below 5 Years of Age
Introduction: Acute diarrhoea is defined as an abnormally frequent discharge of semisolid or fluid faecal matter from bowel, lasting less than 14 days. Enteropathogens that cause diarrhoea may affect physiology of gut in...
A Novel Stability Indicating RP-HPLC Method for Determination of Sofosbuvir in Bulk and Tablet Dosage
The proposed work was accurate and precise stability indicating RP-HPLC method has been developed and validation of Sofosbuvir, in tablet dosage form. The separation was achieved on a Kromasil C18 (4.6×250 mm, 5µ) column...
A Review on Copious Therapeutic Role of Cissampelos Pareira Linn
Cissampelos pareira Linn, an important medicinal plant, mainly found in tropical and subtropical parts of the world. The plant consists of plentiful isoquinoline alkaloids. C. pareira Linn has profound therapeutic and be...
Study of Effects, Advantages and Disadvantages of Poly Pharmacy in Elderly Patients in a Teaching Hospital of Southeast Asia
Objective: This study was performed to assess and establish prevalence of polypharmacy in geriatric population, to identify adverse drug reactions due to polypharmacy and to investigate whether polypharmacy is an indicat...
Analytical Method for the Simultaneous Estimation of Sitagliptin and Simvastatin using RP-HPLC
A novel, rapid, precise and accurate high performance liquid chromatographic method was developed and validated for the simultaneous determination of Sitagliptin phosphate and Simvastatin in bulk drug and pharmaceutical...