ANALYTICAL STABILITY INDICATING UPLC ASSAY AND VALIDATION USING C18 COLUMN FOR FLUOROMETHOLONE IN PARENTERAL DOSAGE FORM
Journal Title: World Journal of Pharmaceutical and life sciences - Year 2018, Vol 4, Issue 8
Abstract
A pecific, precise, accurate and stability indicating UPLC method is validated for estimation of Fluorometholone in parenteral inhaler dosage form. The method employed, with C18 column (250 ×4.6 mm id)—ACE Generix in gradient mode, with mobile phase of Methanol–water (62 : 38 v/v). The flow rate was 1.5 ml/min and effluent was monitored at 240nm. Retention time was found to be 5.215±0.005 min. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) etc. in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 20- 100μg/ml respectively. The LOD and LOQ values for were found to be 0.3245 (μg/ml) and 0.983 (μg/ml) respectively. No chromatographic interference from excipients and degradants were found. The proposed method was successfully used for estimation of Fluorometholone in parenteral dosage form.
Authors and Affiliations
Mohd Shafi
Keywords
Related Articles
- EP ID EP656026
- DOI -
- Views 173
- Downloads 0
EFFECT OF SEED AGE, VARIETIES AND INTRA ROW SPACING ON YIELD OF SOYBEAN (GLYCINE MAX (L.) MERRILL) IN DANGUR DISTRICT, METEKLE ZONE, WEST SOUTHERN ETHIOPIA
The importance of seed age, varieties and spacing on soybean seed yield and quality has not been established in Ethiopia. This study was conducted in Dangur District at Pawe Agricultural Research Site with the objective...
HAS HAART WON HEART OF HIV/AIDS PATIENTS?
Background: The Human Immunodeficiency Virus (HIV) has changed from life threatening to chronic condition due to the almost universal use and accessibility of antiretroviral treatment (ART) among HIV patients. Antiretrov...
LEVELS BCL-2, P53 IN BLOOD SERUM IN SMOKERS (HEALTHY AND CANCEROUS PATIENTS).
One of the levels protection of cells from negative effects of smoking is process apoptosis the cells contacting with cigarette smoke and change in the content of proteins regulators apoptosis. Smokers are at risk the in...
INSILICO DESIGN AND MOLECULAR DOCKING STUDIES OF NOVEL 1, 2, 4- TRIAZOLE DERIVATIVES AS CYP-51 INHIBITORS
Molecular docking has become an increasingly important tool for drug discovery. It can be used to model the interaction between a small molecule and a protein at the atomic level, which allow us to characterize the behav...
A CRITICAL UNDERSTANDING OF LOKA PURUSHA SAMYA VADA IN MAINTAINING THE INTEGRITY OF SUKRAVAHA SROTAS
Vajikarana tantra, the science which deals with the male reproductive system has a very crucial place in the present life scenario. In the fast life of present day, most of the people adopt to junk foods which lack many...