Angiogenesis and Antioxidant Activity of in vitro and in vivo Tuber of Ceropegia pusilla Wight and Arn
Journal Title: Journal of Pharmaceutical Research International - Year 2014, Vol 4, Issue 5
Abstract
Background: Angiogenesis refers to the formation of new blood vessels. This biological process lays an important role in embryonic development. Ceropegia pusilla (Asclepiadaceae) used in folk medicine system to treat various health problems. Present work deals with the analysis of antioxidant and antiangiogenic properties of in vivo and in vitro tuber extracts of C. pusilla by Chicken Egg Chorioallantoic Membrane Assay (CAM) in vivo. Methods: C. pusilla tubers of both in vivo and in vitro were extracted with ethanol and methanol. In vivo anti angiogenesis effect was studied by Chicken Egg Chorioallantoic Membrane (CAM) assay. The antioxidant properties were analysed using 1, -diphenyl-2-picryl- hydrazyl free radical scavenging assay and reducing power activity. Result: Ethanolic extract of both in vivo and in vitro tuber showed higher angiogenic activities 58.3±13.6 and 43.2±12.2 respectively than the methanolic extract. The average number of vessels in in vivo and in vitro ethanolic extracts treated CAM was 6.48 and 5.43. In vivo and in vitro extracts exhibits comparable DPPH radical scavenging activity and better reducing power activity. Conclusion: C. pusilla tuber extracts of both in vivo and in vitro inhibited angiogenesis by blocking normal vascularisation in chick embryo and also having better DPPH and reducing power activity.
Authors and Affiliations
R. Prabakaran, K. Kalimuthu, C. Vani
Keywords
Related Articles
- EP ID EP344931
- DOI 10.9734/BJPR/2014/7481
- Views 75
- Downloads 0
Development and Validation of First Derivative Spectrophotometric Method for Quantification of Darunavir in Tablets
Aims: Darunavir is widely used in HIV/AIDS therapy. It is a HIV protease inhibitor that has excellent efficacy against the virus. The aim of this study is to develop and validate an analytical method fast and free of int...
In vitro Efficacies of Some Nigerian Medicinal Plant Extracts against Toxigenic Aspergillus flavus, A. parasiticus and A. ochraceus
The aim of this work was to find an alternative to chemical fungicides currently used in the control and prevention of activities of toxigenic Aspergillus flavus, A. parasiticus and A. ochraceus in food and feed. The anti...
Antibacterial, Synergistic and β-lactamase Inhibitory Activity of Oroxylem indicum:
Aims: The study was aimed to evaluate the antibacterial, synergistic and β-lactamase inhibitory potential of O. indicum against ampicillin resistant and Extended Spectrum β-lactamase (ESBL) producing bacterial strains. M...
Langmuir, Freundlich, Temkin and Dubinin–radushkevich Isotherms Studies of Equilibrium Sorption of Ampicilin unto Montmorillonite Nanoparticles
Present study was accomplished to prospect the viability of using the montmorillonite (Mon) nanoparticles as an adsorbent to remove the Ampicillin under various experimental conditions. The Physico-chemical characteristi...
Synthesis of Silver Nanoparticles by Sodium Borohydride Reduction Method: Optimization of Conditions for High Anti-staphylococcal Activity
Aim: The aim of this research was to optimize the reaction conditions for the production of silver nanoparticles sufficiently bioactive for incorporation into pharmaceutical gels. Methods: Silver nanoparticles were prep...