Abstract

Objective: To evaluate the effect of inclusion of three levels of anise seed (Pimpinella anisum L.) as an antibiotic growth promoter substitute on growth performance, carcass traits, and immune responses in broiler chickens. Methods: Two hundred and forty, 1-day-old, hatched Ross broilers received a maize-soybean meal basal diet and were allocated randomly in the following five experimental treatments for 6 weeks: basal diet-no additives, basal diet containing 1 g anise/kg diet, basal diet containing 5 g anise/kg diet, basal diet containing 10 g anise/kg diet and basal diet containing flavophospholipol at 4.5 mg/kg diet. At Day 42, two birds per replicate were slaughtered for determination of carcass and organ weights. At Day 28, serum antibody titers against avian influenza virus were measured by the hemagglutination inhibition test. Results: Bodyweight of broilers fed basal diet was higher at 42 d of age than other groups but it was not statistically significant (P>0.05). Broilers receiving basal diet had higher feed intake compared to broilers receiving difference levels of anise seed (P<0.05). The most efficient feed conversion throughout the study was observed in chicks fed diets supplemented with 1 g anise/kg (P<0.05). Most of the carcass characteristics of broilers slaughtered at Day 42 were not influenced by treatments but carcass yield significantly increased (P<0.05) in broilers supplemented with 10 g anise/kg compared to antibiotic group. Antibody titer against avian influenza virus increased in the group treated with 10 g anise/kg diet compared with other groups (P<0.05). Conclusions: The results suggested that dietary inclusion of 10 g anise/kg can be applied as alternatives to in-feed antibiotics for broiler diets.

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