Antagonistic effect of Trichoderma isolates on Sclerotium rolfsii
Journal Title: Journal of Experimental Biology and Agricultural Sciences - Year 2017, Vol 5, Issue 0
Abstract
Present study was carried out with an aim to screen potential Trichoderma strains to control Sclerotium rolfsii. Among the eleven isolates, Seven (T1, T3, T4, T5, T7, T9 and T11) were preliminarily identified as T. harzianum while the rest four (T2, T6, T8 and T10) were identified as T. viride. Further, results of study suggested that isolate T3, T4, T11, T2 and T10 were effective against S. rolfsii. Later, internal transcribed spacer (ITS)-1 sequence analysis confirmed isolate T3 as T. harzianum and T10 as T. viride. In dual culture plate technique, T. harzianum isolate T3 gave highest inhibition of 71.67%, while T. viride isolate T10 stood second with an inhibition of 67.23%. Trichoderma isolate T10 culture filtrate was found to be the most effective in non-volatile antimicrobial compound production. At 15% (v/v) concentration level, it can totally suppress mycelium growth of S, rolfsii. Further, T. viride isolate T2 showed maximum potential in volatile antagonistic compound production with 53.26% radial growth inhibition, followed it with isolate T3 (52.17% of growth inhibition). All the five antagonistic isolates suppressed sclerotial germination and completely killed sclerotia within 20 days; only isolate T11 required 25 days. Isolate T10 was the most effective in germination suppression.
Authors and Affiliations
Subhas Chandra Jana, Malay Mandal
Keywords
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- EP ID EP428114
- DOI 10.18006/2017.5(4).506.514
- Views 106
- Downloads 0
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