ANTAGONISTIC POTENTIAL OF ACTINOMYCETES OF SHARAVATHI ESTUARY, KARNATAKA, INDIA
Journal Title: Journal of Biological and Scientific Opinion - Year 2014, Vol 2, Issue 1
Abstract
Actinomycetes are the most economically and biotechnologically valuable prokaryotes. The study area opted for the work was a splendid estuary in the west coast of Karnataka, Sharavathi estuary of Honnavar. 21 colonies were obtained by dilution plating method of collected samples. All the isolates were tested for antagonistic potential on 12 test pathogens by cross streak method. All the isolates were primarily screened for antibacterial activity against pathogenic bacteria, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Streptococcus species and antifungal activity against C. albicans, C. neoformens, S. cerevisiae, Fusarium and Colletotrichum sp. Cross streaking of test organisms perpendicular to actinomycete growth was carried out in the present investigation and antagonistic activity was determined by noting the inhibition of test organisms. B. subtilis was inhibited by more number of isolates followed by S. aureus, P. vulgaris, P. aeruginosa and S. typhi. E. coli, K. Pneumonia and Streptococcus sp were inhibited by less number of isolates. Significant inhibitory effect was observed in C. albicans and S. cerevisiae which were inhibited by more than 16 isolates. C. lipolytica and C. neoformans were inhibited by 15 isolates.
Authors and Affiliations
Shobha K. S, Seema Patel, Onkarappa R
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