Anti-epileptic drugs inhibit viability of synoviocytes in vitro
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2013, Vol 20, Issue 3
Abstract
Introduction and objective: The hyperplasia of synovial fibroblasts is considered to be essential for the evolution of joint destruction in rheumatoid arthritis (RA). Previously, we reported that anti-rheumatic drugs, both COX inhibitors and disease-modifying anti-rheumatic drugs inhibit proliferation of synoviocytes[i] in vitro[/i]. The presented study investigates the effect of anti-epileptic drugs on the viability and proliferation of synovial fibroblasts [i]in vitro[/i]. Methods: Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. Cell proliferation and viability were assessed by means of BrdU assay and MTT assay, respectively. The IC[sub]50[/sub] value (the concentration of drug necessary to induce 50% inhibition) together with confidence limits was calculated. Results: Carbamazepine inhibited proliferation of human fibroblasts and viability of HIG-82 with IC 50 values of 86 µM and 82 µM, respectively. Diphenylhydantoin, valproate and phenobarbital inhibited viability of HIG-82 cells with the IC[sub]50[/sub] values of 110, 500 and 1031 µM, respectively. Conclusion: Based on these findings, it can be suggested that anti-epileptic drugs may have a disease-modifying effect on rheumatoid synovial proliferation.
Authors and Affiliations
Jolanta Parada-Turska, Patrycja Nowicka-Stążka, Maria Majdan, Mirosław Jabłoński, Waldemar A. Turski, Wojciech Rzeski
Keywords
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