Anti-Inflammatory Activity of Methanolic Fraction of Ethanolic Extract of Crocus Sativus Stigmas in Rats and Mice
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 2
Abstract
Background: Extensive use of anti-inflammatory drugs is associated with undesirable side effects often limiting their use. Hence the search for indigenous safer analgesic agent is always going on. It was considered worthwhile to evaluate anti-inflammatory activity of Crocus sativusethanolic extract methanolic fraction (CSEEMF) in animal models. Methodology: After obtaining permission from animal ethics committee, animals were divided into 5 groups of 6 animals each group like control, standard - Diclofenac 10mg/kg intra-peritoneal or Dexamethasone 0.5mg/kg orally, CSEEMF (200, 400 and 600 mg/kg). Anti- inflammatory activity of stigmas of Crocus sativus was evaluated by using carrageenan induced paw edema, cotton pellet granuloma, formaldehyde induced arthritis in Wistar albino rats and xylene induced ear edema model in Swiss albino mice. Results: Statistical analysis was done by one way analysis of variance followed by Dunnett’s test. p<0.05, p<0.01 and p<0.001 were considered as statistically significant. CSEEMF (200, 400 and 600 mg/kg) revealed anti-inflammatory activity in dose dependent manner in carrageenan induced paw edema model. In cotton pellet granuloma model, CSEEMF (400 and 600 mg/kg P.O) significantly decreased granuloma formation. In formaldehyde induced arthritis model, CSEEMF (200, 400 and 600 mg/kg, intra peritoneally) significantly lowered signs of arthritis. Statistically significant result was also obtained in xylene induced ear edema model with all three test doses however maximum inhibitory results were observed with 600mg/kg dose, when compared with control. Conclusion: CSEEMF showed promising anti-inflammatory activity in dose dependent manner thus CSEEMF may be beneficial in treating inflammatory diseases.
Authors and Affiliations
Heenu Dhar
Keywords
Related Articles
- EP ID EP506019
- DOI -
- Views 45
- Downloads 0
Is prevention and control of cervix cancer a priority amongst future health care providers in India?
Despite being a preventable cancer burden, cervix cancer remains an important public health problem in India. Major reasons behind this are lack of awareness both in the health care providers and community as well as ava...
HRCT Lung in a Follow up Patients of Bone Marrow Transplant with Cough and their Pathological Correlation
Introduction: Bone marrow transplantation is today’s procedure of choice for management of various malignancies and immunologic disorders. HRCT is the common technique in the diagnosis of such recipient’s with respirator...
Perception of First Year MBBS Students on Self-Directed Learning in Anatomy: Improves Concept, Retention and Communication skills
Introduction: As anatomy subject is the basis of all surgical branches, its understanding in depth is of great value. Surgeons need to understand normal morphology of organs to understand the Pathology. Syllabus of Anato...
A Study of Dipstick and Microscopic Analysis of Formed Elements in Urine
Background: Urinalysis is one of the major diagnostic screening tests in the clinical laboratory. Microscopic urine sediment analysis has been the gold standard for decades. Introduction of new technologies and automatio...
Haematological Parameters in Neonatal Sepsis
Background: In neonatal sepsis, the haematological screening parameters and Creactive protein (CRP) have wide variations in performance. Objective: To evaluate the performance of haematological screening parameters and C...