Anti-inflammatory and Anti-infective Properties of Ethanol Leaf and Root Extracts of Baphia nitida
Journal Title: Microbiology Research Journal International - Year 2016, Vol 11, Issue 4
Abstract
Baphia nitida Lodd. (Family Leguminosae-Papilionoideae) is a tropical plant used in African folkloric medicine for the treatment of infections and inflammatory conditions. This study therefore seeks to investigate the biological activities including antimicrobial, antioxidant and anti-inflammatory properties of 70% v/v ethanol leaf and root extracts of B. nitida. The phytochemical constituents of the ethanol root and leaf extracts of B. nitida was determined by thin layer chromatography (TLC) analysis and in vitro phytochemical tests, while their anti-microbial activities were determined using the agar diffusion and micro-dilution methods. The antioxidant and anti-inflammatory activities of this extract was also investigated by the DPPH radical scavenging methods and in vivo carrageenan-induced paw oedema model of inflammation, respectively. The antioxidant properties of the extracts as determined by the DPPH free radical scavenging assay gave IC50 values for reference 𝛼-tocopherol, B. nitida root and leaf extracts as 1.216, 4.656 and 279.4 𝜇g/mL, respectively. The ethanol root extract was further investigated for its anti-inflammatory property. The root extract demonstrated potent anti-inflammatory activity at low concentrations of between 50 - 200 mg/kg body weight as compared to the highest concentration of 400 mg/kg. The extract had a significant decrease in foot weight when compared to the negative control. At 800 mg/kg, significant anti-inflammatory activity was observed with a better reduction in the paw oedema than the positive control (aspirin). The extract also exhibited broad spectrum antimicrobial activities against C. albicans, S. aureus, B. subtilis, P. aeruginosa and E. coli. Combination of antimicrobial, antioxidant and anti-inflammatory properties of the plant may be responsible for its traditional uses in the treatment of infections, wounds and inflammatory conditions.
Authors and Affiliations
Christian Agyare, Somto Oguejiofor, Emelia Oppong Bekoe, Louis Adu-Amoah, Yaw Duah Boakye
Keywords
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- EP ID EP350786
- DOI 10.9734/BMRJ/2016/22459
- Views 85
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