ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 2
Abstract
Objective: The aim of this study was to investigate the capability of Zizyphus spina christi methanol extract to inhibit cancer cell line proliferation.Methods: The leaves of Zizyphus spina christi were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results: The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC50 was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC50 of 33.91 mg/ml.Conclusion: Methanol extract showed potential anti-proliferative activity against RD cell line, which could be due to its antioxidant activity.
Authors and Affiliations
Ahmed R. Abu-raghif, Ghaith A. Jasim, Muneer M. Hanoon
Keywords
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- EP ID EP575856
- DOI 10.22159/ijpps.2017v9i2.15013
- Views 69
- Downloads 0
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