Anti-proliferative Effect of Isoflavones Isolated from Soybean and Soymilk Powder on Lymphoma (DG 75) and Leukemia (CEM) Cell Lines
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 7, Issue 3
Abstract
Aim: Isoflavones in soybean have been linked to reduced risk of some cardiovascular diseases as well as some type of cancers. Extensive epidemiological, in-vitro and animal data suggests lower incidence of cardiovascular disease, hormone-dependent cancers of the breast and prostate, for consumers of soy and soy products. In this study, isoflavones were isolated from Soybean (SB) and soymilk powder (SMP) and the anti-proliferative effects of these isoflavones on Burkitt’s lymphoma (DG-75) and Leukemia (CEM) cell lines were investigated. Place of Study: Department of Clinical Pathology, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra, Ghana between June and December, 2014. Methodology: Isoflavanoids were isolated and HPLC done to confirm the isolate. Isoflavanoids were isolated form SB and SMP and the anti-proliferative effect of isoflavanoid isolated from SB, SMP, pure genistein and daidzein on lymphoma and leukaemia cells were compared using tetrazolium-based colorimetric (MTT) assay. Results: The inhibitory effect of genistein on leukemia (CEM) cell line was stronger (IC50 = 28.4 µM) than daidzein (IC50 = >100 µM). For the DG 75, genistein had a better effect (IC50 = 11.05 µM than daidzein (IC50> 100 µM). The IC50 for SMP were 193.92 µg/ml (DG-75) and 54.17 µg/ml (CEM). In terms of selectivity index (SI), genistein had the highest (>6.95) on CEM followed by genistein (>3.52) and then SB (>1.16). Conclusion: SMP had a better anti-proliferative effect on both CEM and DG-75 compared with SB.
Authors and Affiliations
C. Apprey, F. K. N. Arthur, R. Appiah-Opong, R. A. Annan, I. Tuffour
Keywords
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- EP ID EP344435
- DOI 10.9734/BJPR/2015/18363
- Views 80
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