Anti-Sickling and Membrane Stabilizing Effects of Carica papaya Leaf Extract
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 6, Issue 5
Abstract
The antisickling and membrane stabilizing effect of aqueous extract of Carica papaya leaf was investigated. Fifty confirmed HbSS patients on routine clinical checkup were involved in this experiment after their consent has been obtained. 50 samples of HbSS blood were collected. The samples were divided into five groups, with ten (10) samples in each group. The samples in the different groups were treated with different concentration of the extract ranging from 2mg/ml to 10mg/ml. In group 1 the samples were treated with 2mg/ml concentration of Carica papaya leaf extract. In group 2 the samples were treated with 4mg/ml concentration of Carica papaya leaf extract. In group 3 the samples were treated with 6mg/ml concentration of Carica papaya leaf extract. Group 4 samples were treated with 8mg/ml concentration of Carica papaya leaf extract. While group 5 samples were treated with 10mg/ml concentration of Carica papaya leaf extract. For each sample three different tests were carried out (osmotic fragility, sickling test and reversal sickling test). For each test, two test tubes were set up; control without Carica papaya leaf extract and Experiment with Carica papaya leaf extract. Data obtained was analysed using Student’s T-test on SPSS software computer package. Results showed a dose dependent significant reduction (p<0.05) in sickling of Reb Blood Cell treated with different doses of the extract, also the extract show ability to reverse already sickled Red Blood Cells as there was significant reduction (p<0.05) in sickling of Red Blood Cells after treatment. There was also significant reduction (p<0.05) in osmotic fragility of Red Blood Cells treated with the extract. We therefore conclude that aqueous extract of Carica papaya leaf has anti-sickling effect which may be related to its direct action on HaemoglobinS and also its membrane stabilizing ability. This extract therefore may be a good candidate in Sickle Cell Disease therapy.
Authors and Affiliations
A. O. Naiho, B. C. Okonkwor, C. Okoukwu
Keywords
Related Articles
- EP ID EP347123
- DOI 10.9734/BJMMR/2015/14608
- Views 63
- Downloads 0
Cytogenetics of Leukemias: Identifying Genetic Abnormalities with Clinical and Prognostic Significance
Introduction: The importance of cytogenetics in neoplastic processes such as leukemia is known. In 1914, Theodor Boveri suggested that chromosomal abnormalities were cellular alterations that cause the transition from no...
Internal Medicine as a Career Choice among Rotatory Interns in a Developing Country- A Multi Centre Study
There is shortage of medical manpower including internists in Nigeria. Young interns prefer certain specialties to the neglect of others. This creates a gap in health care delivery. Objectives: The aim of the study was t...
Cardiovascular and Respiratory Responses of Apparently Healthy Participants to Cervical Traction in Sitting Position Using Different Weights
Background and Aim: Cervical Traction (CT) is a vital physiotherapeutic modality in the management of cervical musculoskeletal disorders. This study determined the cardio-respiratory responses to CT using different perce...
Acute B-Cell Lymphoblastic Leukemia/Acute B-Cell Lymphoblastic Lymphoma Presenting as Bilateral Breast Masses
Aims: Metastases of leukemia and lymphoma to both breasts were very rare. We have aimed to report a rare and interesting case that presented with metastases of leukemia and lymphoma to both breasts. Case Report: A thirty...
Impact of Nutrient Intake and Physical Activity Level on Nutritional Status among University Students in Ghana
Aim: This study assessed the impact of nutrient intake and physical activity level on nutritional status of undergraduate university students Study Design: Cross sectional study design. Place and Duration of Study: Scho...