Anti-viral Effect and Mechanism of Carvacrol on Herpes Simplex Virus Type 1

Journal Title: International Journal of Medical Laboratory - Year 2018, Vol 5, Issue 2

Abstract

Background and Aims: Herpes simplex virus type 1 (HSV-1) belonging to herpes viridae family is a normal human pathogen with benign lesions in immunocompromised patients which creates serious problems. Acyclovir is used to treat herpetic infections. Because of developing drug-resistant strains, the use of medicinal plants and their related compounds with fewer side effects is considered for the treatment of patients. In this study the antiviral effect and mechanism of carvacrol, a medicinal compound, on HSV1 virus was studied. Materials and Methods: In this study, the maximum nontoxic concentration on vero cells was determined by MTT. The antiviral effect of the compound was determined by TCID50. The expression of early and late stages of viral replication (UL52 and UL27) was evaluated by real time polymerase chain reaction method. Results: The toxic concentration of carvacrol causing 50% cell death was 0.001%. The mechanism of action of the compound showed that pretreatment of HSV-1 with carvacrol prior to infection inhibits its ineffectivity approximately to 70%. The results also showed no reduction in the early and late gene expression of herpes virus replication. Conclusions: Overall, the findings demonstrated that the carvacrol has inhibitory effect on HSV-1 by direct inhibition of free virus particles.

Authors and Affiliations

Mina Kamalabadi, Akram Astani, Fahimeh Nemati

Keywords

Related Articles

Effects of Thymol on Serum Biochemical and Antioxidant Indices in Kindled Rats

Background and Aims: Kindling is regarded as an experimental model of temporal lobe epilepsy that reflects a process of progressive and persistent intensification of seizure. Experimental and clinical evidence suggest th...

The Peep of Nanotechnology in Reproductive Medicine: A Mini-review

Nanotechnology has opened a new field in medicine as well as in other sciences. The aim of this study was to seek the capability of nanotechnology for the treatment of various reproductive diseases. In this study, we ana...

Investigating the Potential Role of piRNAs in Male Infertility

Infertility is a reproductive-related disorder and different factors account for its development. Male factors contribute to at least 20% of infertility. Despite the efforts performed in this field, the causes often rema...

Comparative Effect of Cinnamon Essential Oil, Diclofenac and Morphine on Acute and Chronic Pain in Mice

Backgrounds and Aims: Cinnamomum Zeylanicum is a medicinal herb used in Iranian traditional medicine as an analgesic spice, which its analgesic effect has been experimentally confirmed. Thus, this study was conducted to...

Advances in Hematopoietic Stem Cell Mobilization and Peripheral Blood Stem Cell Transplantation

Hematopoietic stem/progenitor cells (HSPCs) which give rise to different blood cell types are present within the bone marrow microenvironment, especially in flat bones such as skull, vertebrae, pelvis and chest. Interact...

Download PDF file
  • EP ID EP299190
  • DOI -
  • Views 96
  • Downloads 0

How To Cite

Mina Kamalabadi, Akram Astani, Fahimeh Nemati (2018). Anti-viral Effect and Mechanism of Carvacrol on Herpes Simplex Virus Type 1. International Journal of Medical Laboratory, 5(2), 113-122. https://europub.co.uk/articles/-A-299190