Antiangiogenetic Effect of Neem Root Extract on the Fin of Zebrafish (Fin Regeneration Method)
Journal Title: Journal of Medical Science And clinical Research - Year 2014, Vol 2, Issue 11
Abstract
Cancer is a serious cause of death globally. Cancerous cells cannot be distinguished easily from the normal cells. Rapid development of cancerous cells depends on the process of Angiogenesis. Inhibition of the angiogenesis is a novel strategy in cancer treatment. Currently, herbal medicines are also being used, along with chemotherapy to reduce the side effects. For the present study, Neem was selected as it is a versatile tree in India. From the ancient times it has been considered as a mighty tree which gives good health. Roots of that tree were taken to study anti-angiogenesis activity on Zebra fish. The different parts of Neem tree were proven for their cytotoxic activity; hence water soluble fractions of crude methanolic extract of neem roots were taken as a test drug. Fin Regeneration in Zebra fish was selected as an experimental model. Fish were divided into groups, each with six fish. Fins were amputated, allowed to grow for 15 days. They were treated with Different concentrations of the test drug and standard drug. Length of the regenerated fin was measured frequently using digital microscope, and different software. Dose response was calculated for the test drug in comparison with standard drug and control groups. A graph of dose response vs. concentration was plotted accordingly. The test drug has shown desirable effect in angiogenesis inhibition.
Authors and Affiliations
L. P. B. Lavanya Uppuluri
Keywords
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