Antiangiogenic and Apoptotic Effects of Oleuropein on Breast Cancer Cells
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 16, Issue 4
Abstract
Oleuropein is a fundamental polyphenol contained in olive fruit, oil, and leaf, with a bitter taste. A decrease in its level is observed as the fruit ripens. Leaves of oil tree were used in this study as a possible origin of oleuropein. In this study, cytotoxic, genotoxic, apoptotic and antiangiogenic impacts of isolated oleuropein (0.5xIC50, IC50 and 2xIC50) on MCF-7 human breast adenocarcinoma cells were examined by means of 3-[4,5- Dimethylthiazol-2-yl] 1-2,5-diphenyltetrazolium bromide (XTT) kit, Comet assay, Poly (ADP-Ribose) Polymerase (PARP) cleavage ELISA Kit and Vascular Endothelial Growth Factor (VEGF) Human ELISA Kit for 24, 48 and 72 h, respectively. The IC50 value of oleuropein on MCF-7 cells was found to be 11.02±0.52, 9.43±0.41 and 6.81±0.18 µg/mL for 24, 48 and 72 h, respectively. The time and concentration-related cytotoxic impacts of oleuropein were determined to be significant. Cleaved PARP level was significantly increased especially with an increase in the concentration of oleuropein. However, a significant decrease in VEGF level was observed (p<0.05). Furthermore, oleuropein increased damage on DNA for 48 and 72 h. Findings showed oleuropein as a potential antiangiogenic, apoptotic and genotoxic agent for the breast cancer cells.
Authors and Affiliations
Recep Liman, Funda Karabağ Çoban, Ibrahim Hakkı Ciğerci, İbrahim Bulduk, Selahattin Bozkurt
Keywords
Related Articles
- EP ID EP312960
- DOI 10.9734/BJPR/2017/33403
- Views 112
- Downloads 0
Evaluation of the Physicochemical and in vitro Dissolution Properties of Metformin Hydrochloride Tablet Brands Marketed in Five Cities in Ghana
Aim: A comparative evaluation of the physicochemical and in vitro dissolution properties of metformin hydrochloride tablet brands sampled in five cities in Ghana was undertaken to assess their pharmaceutical equivalence....
Development and Validation of UV Spectrophotometric Method for the Estimation of Haloperidol
Aims: The aim of the present work was to develop and validate a sensitive, simple, accurate, precise & cost effective UV spectrophotometric method for the estimation of haloperidol in prepared pharmaceutical formulations...
Fenofibrate Potentiates the Antihyperglycemic, Antidyslipidemic and Hepatoprotective Activity of Pioglitazone in Alloxan-Induced Diabetic Rats
Aim: The present research was designed to investigate the hypoglycemic, hypolipidemic and hepatoprotective activity of the fixed dose combination of pioglitazone and fenofibrate. Methodology: For the study purpose, piogl...
Spectroscopic Characterization and Antibiotic Study of Novel Compound Extracted from Curcuma longa (Turmeric)
Aims: To isolate and investigate the antibacterial potency of novel compound obtained from methanolic extract of Curcuma longa rhizome. Place and Duration of Study: Bio-inorganic and Materials Chemistry Research laborato...
Biochemical Effects of Dietary Consumption of Raw and Fermented Seeds of African oil Bean (Pentaclethra macrophylla Benth) in Rats
Aim: Studies have reported the microbial role, biochemical changes and nutritional quality of fermented African oil bean seed, known as Ugba in the eastern Nigeria; however, assessment of possible health impact of this f...