ANTIBACTERIAL ACTIVITY OF FLOWER HEADS OF WEDELIA TRILOBATA (L.) A. S. HITCHC
Journal Title: Journal of Biological and Scientific Opinion - Year 2014, Vol 2, Issue 6
Abstract
Present study is conducted to analyse the antibacterial activity of flower heads of Wedelia trilobata against pathogenic bacterial strains and to analyse phytochemical background of various extracts. Fresh flower heads collected from Kerala, India. Air dried flower heads were extracted successively in petroleum ether, chloroform, acetone, ethanol and water. Preliminary antibacterial activity was analysed by disc-diffusion method and further confirmed by MIC and MBC. Preliminary detection of phytochemicals was done. Among the ten bacterial strains nine showed considerable inhibition of growth towards acetone extract. Acetone extract selected for detailed antibacterial evaluation tests like minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC). The MIC and MBC of floral extracts tested against one of the most sensitive strains, Streptococcus haemolyticus. MIC and MBC values were 25 mg/ml and 50 mg/ml respectively. Preliminary phytochemical evaluation revealed the presence of flavonoids, phenolics and terpenoids in active acetone extract. The present investigation showed the effectiveness of crude extract of this plant against tested bacterial strains. The presence of potential phytochemicals like phenolics, flavonoids and terpenoids in active acetone extract might be one of the reasons for its antibacterial property.
Authors and Affiliations
Rajalakshmi Shankar , Toji Thomas
Keywords
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- EP ID EP121918
- DOI 10.7897/2321-6328.02692
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