Antibacterial Activity of Lactobacillus spp and Lactococcus spp Isolated from Various Parts of Pebbly Fish, Alestes baremoze
Journal Title: Microbiology Research Journal International - Year 2016, Vol 17, Issue 2
Abstract
The study sought to identify microbial communities and potential probiotics in one of the popular fish species, Alestes baremoze. A total of 15 samples were collected from Lake Albert and swabs of the skin, gills and gut were made, and cultured on general purpose and selective media. The bacteria isolated were confirmed using morphological and biochemical tests while probiotic screening was done using the agar spot method. The prevalent potential pathogenic bacteria were Staphylococcus aureus (100%) and Escherichia coli (67.7%). The highest total microbial load was generally recorded for samples from the skin. Staphylococcus spp had the highest total microbial load recorded from all the samples (skin, 8.50±22.1x102 cfu/g and gills, 8.00±24.3x102 cfu/g). When the effect of potential probiotic genera (Lactobacillus spp and Lactococcus spp) isolated from skin, gills and gut was investigated, Lactobacillus spp showed the highest overall activity against all test pathogens. The highest activity for Lactobacillus spp was demonstrated against E. coli (15.4±0.19 mm) and S. aureus (14.0±0.17 mm). The highest activity for Lactococcus spp was demonstrated against S. aureus (8.7±0.12 mm) and E. coli (7.2±0.1 mm). Furthermore, no probiotic activity was recorded against Streptococcus spp and Pseudomonas spp. No significant (p> 0.05) differences in antimicrobial activity were noted using Lactic acid bacteria isolated from the different fish body parts. Based on the positive results from this study, Lactobacillus spp. should be further characterised and studied in challenge experiments in fish to explore its probiotic effects.
Authors and Affiliations
Charles D. Kato, Carol E. Kahuma, Victoria T. Namulawa, Nasser Kasozi
Keywords
Related Articles
- EP ID EP350746
- DOI 10.9734/BMRJ/2016/28820
- Views 74
- Downloads 0
Nocardiopsis sp. 5 Endophytic to Tulsi Leaves – Isolation and Antimicrobial Activity
Aims: To isolate and identify the endophytic Nocardiopsis sp.5 from Tulsi (Ocimum sanctum) leaves and estimates its antimicrobial activity against various human pathogens and tea pathogens. Study Design: Rare endophytic...
Application of a Novel Tongue Resin Replication System for in vitro Biofilm Studies
Aims: Biofilms present a strategy for microorganisms to survive and resist adverse conditions. In order to further our understanding of this resistance mechanism, it is of much importance to develop new methodologies in...
Latent Toxoplasmosis is Not a Risk Factor for Pregnancy-induced Hypertension
Aims: To compare between Toxoplasma IgG antibody seroprevalence in pregnancy-induced hypertensive females (cases) versus normotensive pregnant females (control), and to identify potential risk factors in Toxoplasma infec...
Antibacterial Potential of Different Parts of Aerva lanata (L.) Against Some Selected Clinical Isolates from Urinary Tract Infections
Aims: To investigate the antibacterial activity of different parts of Aerva lanata against Staphylococcus saprophyticus, Streptococcus agalactiae, Acinetobacter baumannii, Xanthomonus citri, Klebsiella pneumoniae and Pro...
Smokeless Tobacco – Ammunition against Dental Caries? A Microbial Study
Aims: The tobacco plant, Nicotiana tabacum, has been one of the major risk factors for oral cancer. However, the literature has endowed it as “holy herb” since the pre-Columbian era as being used for treating pain, poiso...