Antibacterial Activity of Ocimum gratissimum L. against Some Selected Human Bacterial Pathogens
Journal Title: Journal of Pharmaceutical Research International - Year 2017, Vol 20, Issue 1
Abstract
Aim: This study was designed to investigate the bioactive spice to determine its activity against some selected human bacterial pathogens. The spice studied was Ocimum gratissimum (clove basil). Place and Duration of study: Microbiology laboratory of Bells University of Technology between October, 2016 to July, 2017. Methodology: The plant extracts was tested for their activity against Staphylococcus species, Escherichia coli, Enterococcus faecalis, Proteus mirabilis and Klebsiella pneumoniae collected from microorganism bank of Bells University of Technology. Antibacterial activity was carried out using agar diffusion method while agar dilution method was employed for determining the minimum inhibitory concentration (MIC) on Mueller-Hinton agar. Results: The findings of this study showed that the spice contained phytochemicals which made it active against the tested microorganisms. Hot water extract was more active against all tested isolates with MIC of 5.0 mg/ml against Klebsiella pneumoniae to 10 mg/ml against Escherichia coli, Proteus mirabilis, Staphylococcus aureus and Enterococcus faecalis. Zone of inhibition was between 18 mm for Staphylococcus aureus and Enterococcus faecalis and 31 mm for Proteus mirabilis. Conclusion: Ocimum gratissimum has a broad spectrum antibacterial activity against all tested isolates and thus has a potential as a source of natural drugs. However, in vivo studies are recommended.
Authors and Affiliations
Ajose Daniel Jesuwenu, Okozi Ogheneoremume Michael
Keywords
Related Articles
- EP ID EP313130
- DOI 10.9734/JPRI/2017/37897
- Views 109
- Downloads 0
Development and Validation of Bio Analytical Method for Estimation of Bortezomib in k3 EDTA Human Plasma Using HPLC-ESI-MS/MS and Its Application to a Bioequivalence & CME Studies
Purpose: To develop a highly selective, reproducible & precise rugged bio analytical method for estimation of Bortezomib (BTZ), “A Protease Inhibitor” in human plasma by validating the developed method in accordance to U...
Use of Antimicrobial Medicines among University Students in Sierra Leone
Aims: Misuse of antimicrobial medicines is a major contributory factor to development of resistant strains of micro-organisms, therapeutic failure and increased healthcare costs in many countries. To identify pattern of...
TiCl4/nano-sawdust as an Efficient Biocatalyst for the Synthesis of Highly Substituted Dihydro- 2-oxopyrroles as Antimicrobial Agents
Aim: Sawdust nano particles was prepared from the pine wood and applied for the synthesis of TiCl4/nano-sawdust as a readily available, inexpensive, biodegradable and environmentally benign heterogeneous solid acid bioca...
The Protective Effect of Calcitriol on Diabetic Neuropathy in STZ-induced Diabetic Rats
Diabetic peripheral neuropathy (DPN) is one of the diabetic complications that have a complex etiology and even with good glycemic control can have severe consequences. To date, there is no effective treatment for DPN. T...
Anti-oxidant and Antimicrobial Flavonoid Glycosides from Alstonia boonei De Wild Leaves
Aims: Alstonia boonei De Wild (Apocynaceae) leaves are used traditionally in several parts of West Africa (including Nigeria) and Asia for the treatment of various ailments such as rheumatic and muscular pains as well as...