Antibacterial Activity of Sida cuneifolia Vollesen against Staphylococcus aureus
Journal Title: Journal of Advances in Microbiology - Year 2017, Vol 7, Issue 1
Abstract
Background: Sida cuneifolia is utilized traditionally to treat many ailments yet as far as we know its medicinal properties have not been scientifically tested locally in Kenya. The aim of the present study was therefore to investigate the antimicrobial properties of S. cuneifolia against Staphylococcus aureus and investigate the phytochemicals present in the leaves, roots and stem that are of medicinal importance. Method: The plant was separated into root, leaves and stem bark. Water and ethanol were used for extraction of active ingredients. Antimicrobial bioassays and minimum inhibitory concentration (MIC) tests were done on S. aureus. Phytochemicals of medicinal importance were also determined using thin layer chromatography. Results: Ethanol extracts had significantly higher activity than water. Roots showed higher inhibition than leaves and stem. The stem ethanol extracts had an MIC of 10-10 g/ml. Ethanol leaf and root extracts had all the five phytochemicals tested for (alkaloids, flavonoids, tannins, saponins and terpenoids). Alkaloids were absent in ethanol stem extracts while both alkaloids and flavonoids were absent in the stem and leaf water extracts. Conclusion: The results showed that the S. cuneifolia leaf and root ethanolic extracts could be used to treat ailments caused by S. aureus. It is recommended that further toxicological testing be done.
Authors and Affiliations
B. Kipng’etich, L. A. Mwamburi, J. M. Mulei, P. Jeruto, T. Chemweno
Keywords
Related Articles
- EP ID EP338287
- DOI 10.9734/JAMB/2017/37894
- Views 63
- Downloads 0
Effect of Alkaline Treatment on Nutrient and Antinutrient Contents of Mucuna pruriens (L.) DC.
Aims: To investigate the impact of Trona and woodash (alkaline tenderizers) on aniti-nutrient and nutrient content of Mucuna pruriens during fermentation. Study Design: Natural fermentation and fermentation using additiv...
Antimicrobial Efficacy of Some Medicinal Plants on Different Bacterial Isolates Associated with Semen of Infertile Men
Infection of the male genitourinary tract represents a significant health care problem and account for almost 15% of cases of male infertility. The present study aims to isolate and identified different bacterial isolate...
Preliminary Investigation of Bio-preservative Effect of Cola millenii Extracts on the Shell-Life of “Kunu-Zaki”
Aim: The aim of this study was to investigate the potentials of Cola millenii seed and pulp extracts as a possible bio-preservative agent for kunu-zaki, a Nigerian fermented beverage. Methodology: The plant materials wer...
Microbiological Analyses of Kunu Drinks Locally Produced and Sold in Calabar, Southern Nigeria
Kunu is a non-alcoholic Nigerian beverage produced locally from cereals. The method of preparation, as well as the key ingredients and spices added as flavoring agents are always not standardized. This study was carried...
Physicochemical Analysis and Microbiological Assessment of Tannery Effluent Discharged from Tanneries around Nigeria’s Kano Industrial Estates
Aims: Research study on physicochemical analysis and microbiological assessment of tannery effluent discharged from tanneries around Nigeria’s Kano industrial estate was carried out. Duration of Study: The study was co...