Antibacterial, Cytotoxic and Antioxidant Potential of Vitex Negundo Var. Negundo and Vitex Negundo Var. Purpurascens – A Comparative Study
Journal Title: STAR Journal - Year 2013, Vol 2, Issue 3
Abstract
The present study was carried out to estimate the content of total phenolics, flavonoids, negundoside and agnuside and to determine antibacterial, cytotoxic and antioxidant efficacy of methanol extract of two varieties of Vitex negundo namely V. negundo var. negundo (Vnvn) and V. negundo var. purpurascens (Vnvp). Total phenolic and flavonoid content of leaf extracts were estimated by Folin-Ciocalteau reagent and Aluminium chloride colorimetric estimation method respectively. Negundoside and agnuside content were estimated using HPLC. Antibacterial activity of both the extracts was tested by Agar well diffusion assay. Cytotoxicity of extract was evaluated by Brine shrimp lethality bioassay. Antioxidant activity of extracts was determined by DPPH free radical scavenging and Ferric reducing assay. The content of total phenolics and flavonoid were higher in extract of Vnvp. Negundoside and agnuside contents were high in Vnvn and Vnvp respectively. Leaf extract of Vnvp was shown to display marked antibacterial, cytotoxic and antioxidant activity when compared with extract of Vnvn. Both the varieties of V. negundo showed marked antibacterial, cytotoxic and antioxidant activity. The extract of Vnvp displayed marked bioactivities than that of extract of Vnvn. The presence of high phenolic and flavonoid content could be attributed to the marked bioactivities of Vnvp.
Authors and Affiliations
Prashith Kekuda TR| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Manasa M| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Poornima G| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Abhipsa V| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Rekha C| Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road, Shivamogga-577201, Karnataka, India, Shivaleela P. Upashe| College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia, Raghavendra HL| College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia
Keywords
Related Articles
- EP ID EP9573
- DOI http://dx.doi.org/10.4314%2Fstar.v2i3.98737
- Views 368
- Downloads 20
Evaluation of Bread Wheat (Triticum aestivum L.) Breeding Lines for Yield and Yield Related Characters in Horo Guduru Wollega Zone, Western Ethiopia
A total of thirty-six bread wheat (Triticum aestivum L.) breeding lines were evaluated for yield and yield related traits at high and midland environments of Horo Guduru Wollega Zone, western Ethiopia during 2013 croppin...
Development of New Formulation for Mouth Disintegrating Tablets of Metoclopramide by Sublimation Method
The main purpose of the present research work was to mask the bitter taste of the active drug and to formulate the MD tablets by sublimation method. Metoclopramide HCl tablets were prepared by masking the bitter taste...
Broadband: An Insight and Its Benefits
Countries around the world have nominated broadband network as establishing an information society for achieving their social, economic and scientific goals. Broadband has been a beacon for investors and a stimulus for...
Reading Urban Environment by Photo: A Critical Tool for Socio-Cultural Analyzing
Photography has been used as a research tool throughout a range of disciplines. In the last decades usage of photos in landscape and urban design grow noticeably; yet applying it in architectural research or education...
Effects of Different Feeding Regimes on Leather Quality of Finished Blackhead Ogaden Sheep
No Abstract