ANTIBACTERIAL EVALUATION OF METHANOLIC RHIZOME EXTRACT FROM AN IN VIVO AND IN VITRO GROWN PTERIDOPHYTE, DRYNARIA QUERCIFOLIA (LINN.) J SMITH
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2015, Vol 8, Issue 4
Abstract
ABSTRACTObjective: Drynaria quercifolia (Linn.) J. Smith has been used in many herbal folklore medicines in Ganjam - Gajapati districts of Odisha state.Study pertains to its in vivo and in vitro antibacterial activities were undertaken to scientifically validate one of such therapeutic claim raised in theliteratures, foreseeing the vulnerable state of the species.Methods: Methanolic extract from both the in vivo grown plant and in vitro grown callus were prepared and tested for their antibacterial efficacyagainst a wide range of bacterial pathogens concerned. Both agar well diffusion and disk diffusion methods were followed for evaluation ofantibacterial activities. Development of callus was done using modified MS media supplemented with 20/gl sucrose and varied concentrations ofauxins and cytokinins and the extract was prepared thereof. Antibacterial potency of both the extracts was measured in terms of zone of inhibitionand statistical analysis was performed using Graph pad prism 6 software.Results: Methanolic extracts from both in vivo and in vitro samples had broad spectrum antibacterial activity on series of bacteria; however, bacterialspecies like Salmonella typhi, Shigella flexinneri, Streptococcus pneumoniae were resistant to both the samples. Further, the in vitro sample showed ahigher degree of inhibition compared to the in vivo sample, was revealed.Conclusion: Study explored that the plant species is a potential source of antibacterial activity suggesting its in vitro culture for conservation and toobtain higher degree of antibacterial efficacy as well.Keywords: In vitro culture, Therapeutic claim, Folklore medicine, In situ/ex-situ conservation.INTRODUCTION
Authors and Affiliations
Ranjan Padhy, Santosh Kumar Dash
Keywords
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