Antibacterial Potential of Magnesium Oxide Nanoparticles Synthesized by Aspergillus niger
Journal Title: Biotechnology Journal International - Year 2017, Vol 18, Issue 1
Abstract
A total of 280 urinary tract infection samples were collected in this investigation. Out of them 212(75.7%) samples showed a positive response.to bacterial isolates. Morphological, cultural and biochemical testes were confirmed using VITEK 2 System. Its revealed that 54 (30.2%) of the bacterial isolates were gram positive and 158 (69.8%) gram negative. The bacterial isolates were distributed as Escherichia coli 96 (45.2%), followed by Klebsiella pneumonia 48 (22.6%), Staphylococcus aureus 43(20.3%), Pseudomonas aeruginosa 14 (6.6%), and Staphylococcus epidermidis 11 (5.2%). The synthesis of magnesium oxide nanoparticles (MgO NPs) was performed using Aspergillus niger Method. Agar Wells Diffusion Method was applied for the evaluation of antibacterial activity against gram positive S. aureus, and gram negative P. aeruginosa bacteria isolated from Urine tracts infection (UTI). The results showed that the biosynthesized MgO NPs appeared to be an extracellular with a size range of 43-91 nm as confirmed by Scanning Electron Microscopy (SEM) and UV-Visible spectroscopy for the absorbance band at 256.5 nm. Besides, MgO NPs were found to be an effective antibacterial agent against Gram positive S. aureus, and the zone inhibition diameter at 27 mm. Howover, the zone inhibition diameter against gram negative P. aeruginosa bacteria was at 24 mm, compared with inhibition effects of ciprofloxacin antibiotics at 24 and 20 mm, respectively.
Authors and Affiliations
Emad J. Ibrahem, Karkaz M. Thalij, Amin S. Badawy
Keywords
Related Articles
- EP ID EP315110
- DOI 10.9734/BJI/2017/29534
- Views 111
- Downloads 0
Purification and Characterization of α-Glucan Phosphorylase Isoform Pho 2 from Spinach Leaves
α-Glucan phosphorylase is an important enzyme of carbohydrate metabolism. In spinach leaves, it has been reported in two multiple forms viz. Pho 2 (cytosolic) and Pho 1 (plastidial). Here, we extracted and purified Pho 2...
Application of Box-Behnken Design for the Optimized Production of Lactic Acid by Newly Isolated Lactobacillus plantarum JX183220 Using Cassava (Manihot esculenta Crantz) Flour
Aim: The present study aimed at optimization of Lactic acid production using new isolate, Lactobacillus plantarum JX183220 with cassava flour (Manihot esculenta Crantz) in semi-solid fermentation by Response Surface Meth...
Effect of Cooking Time on Starch and Cyanide Contents of Freshly Harvested Cassava Tubers Used for Tapioca Production
This study investigated the effect of cooking time on starch and cyanide contents of freshly harvested cassava tubers used for tapioca production. Tapioca is a cassava meal commonly consumed in most part of the world and...
Antibacterial Potential of Magnesium Oxide Nanoparticles Synthesized by Aspergillus niger
A total of 280 urinary tract infection samples were collected in this investigation. Out of them 212(75.7%) samples showed a positive response.to bacterial isolates. Morphological, cultural and biochemical testes were co...
Fungal Infection Enhances Anthocyanin Production in Blue Flowers of Clitoria ternatea
Aims: A white fungus which commonly infects blue petals of Clitoria ternatea L. (CT) was identified and total anthocyanin content and antioxidant activity of CT extracts from infected and uninfected CT were compared. St...