Antibacterial Susceptibility Evaluationof Enteric Bacteria from Stool Samples of Diarrheal Patients in Awka metropolis.
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 3
Abstract
:This study was carried out in order to isolate enteric bacteria from stool of diarrheal patients and determine their antibacterial susceptibility profile. Fresh diarrheal stool samples were collected from four Hospitals in Awka, Anambra state, transported to the laboratory, and maintained in the refrigerator at 4o c for microbial examinations. The samples were inoculated onto Eosin Methylene Blue agar and MacConkey agar at room temperature for 24hours. Seven (7) isolates of six genera were isolated and identified as Salmonella spp., Shigella spp., Campylobacter spp., Esherichia spp., Staphylococcus spp. and Vibrio spp. Salmonellaspp. was the most frequently isolated bacteria. Antibacterial susceptibility test of the isolates to conventional antibiotics and aqueous extract of Thymus vulgaris leaves were performed. The results showed that the isolates were susceptible to the antibiotics at various concentrations. Septrin (30ug) had the highest zone of inhibition of 35mm on Salmonella spp. whereas Gentamycin (10ug) had the lowest zone of inhibition of 10mm on Vibrio spp. The antibacterial susceptibility test of the isolates to aqueous extract of leaves of Thymus vulgaris at 1000, 500 and 250mg/ml concentrations showed that the higher the concentration, the greater the zone of inhibition. The zones of inhibition ranged from 14 – 32mm.Though the extracts were in the same range of effectiveness on the isolates as the conventional antibiotics at higher concentrations, it is still suggested that more research be done on the use of natural agents like extracts of Thymus vulgaris in the treatment of diarrhea to discourage the use of synthetic antibiotics.
Authors and Affiliations
ChidiOnuorahLilian C, OkaforUgochukwu C, OkoroNnenna C. N, Anyaoha VictoriaI, Kyri an-OgbonnaEvelynA, Onuorah MargaretE, ,ChiemelaVictor O
Keywords
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- EP ID EP395405
- DOI 10.9790/3008-1303041218
- Views 87
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