Antibiofilm activity of aminopropanol derivatives against Pseudomonas aeruginosa
Journal Title: Фармацевтичний журнал - Year 2017, Vol 1, Issue 1
Abstract
Bacterial biofilm, particularly formed by Pseudomonas aeruginosa, are a cause of severe chronic infectious diseases. Bacteria within a biofilm are phenotypically more resistant to antibiotics and the macroorganism immune system, making it an important virulence factor for many microbes. The aminopropanol derivatives with adamantyl (KVM-97) and N-alkylaryl radicals (KVM-194, KVM-204, KVM-261, and KVM-262) were used as study object. The aim of this study was to investigate the antibiofilm activity of compounds on biofilm formation and on mature biofilm of P. aeruginosa. The effects of the aminopropanol derivatives on the biofilm mass were evaluated by using crystal violet assay. Ciprofloxacin, meropenem, ceftazidime, gentamicin were used as reference substances. Reported results demonstrate that all compounds displayed antibiofilm activity at the tested concentrations. Remarkable reduction in biofilm formation of P. aeruginosa was found after treatment with KVM-97, KVM-261 and KVM-262 in high concentration (5× MIC), biofilm inhibition activity were 84.3%, 90.5% and 83.3% respectively. After a treatment with KVM-204 at 250 μg/ml (5× MIC) 76.6% of the preformed 24-hr biofilms were destroyed. Furthermore, compounds KVM-97, KVM-194, and KVM-261 in both concentrations showed potent antibiofilm activity against the P. aeruginosa, inhibition activity values being between 56.7 and 65.7%. All tested compounds in dose-dependent manner exhibited pronounced inhibition activity against mature 5-days P. аeruginosa biofilm. It was also observed that tested compounds show high antibiofilm activity in comparison to reference antimicrobials. The aminopropanol derivatives may provide templates for a new group of antimicrobial agents and potential future therapeutics for treating chronic infections.
Authors and Affiliations
D. M. Dudikova, Z. S. Suvorova, V. V. Nedashkivska, A. O. Sharova, M. L. Dronova, N. O. Vrynchanu
Keywords
Related Articles
- EP ID EP554113
- DOI 10.32352/0367-3057.1.17.12
- Views 49
- Downloads 0
Cariesprophylactic and periodontoprotective action of octenidine hexafluorosilicate in rats obtained with cariesogenic ration
In recent years, ammonium hexafluorosilicate and hexafluorosilicates of organic amonium cations are actively studied, which demonstrate certain advantages over traditional compounds of fluorine. Previously, it was shown...
The study of acid-base properties of 5-R-4-R1-1,2,4-triazole-3-thio(sulfo)acetic acids
Working on synthesis of 1,2,4-triazole-3-ylthioacetic acids and also salts, complex esters, amides, hydrazides, ylidene- and diacyl hydrazides, based on them, we see that the study of physical and chemical properties of...
Study of optimal parameters of extrusion for creation liposome with irinotecan
Today, the development of drug delivery systems is focused on creating of products with improved pharmacological efficacy and safety of action for the patient. Therefore, the development of systems such as liposomes, emu...
Study of the base influence on the foaming properties gel shampoo with minoxidil
Alopecia causes a considerable psychological discomfort for the people and so it offers a great medical and social problem. Minoxidil has stayed practically the only medication with certainty recommendation for the recov...
Problems of scientific substantiation of extemporaneous formulations technologies and ways of its solution. P a r t IІ. Comparative analysis of modern pharmacopoeial requirements to technology and quality control of extemporaneous medicines
For today almost all countries of the world have State Pharmacopoeias, which are collections of standards for medicines and provide the basic principles of preparing dosage forms. They have legislative character, are man...