Abstract

Objective: To evaluate the antimicrobial activity of different solvent extracts of the species Thais bufo against the Streptococcus pyogenes and Staphylococcus aureus. Methods: Different types of solvents were used such as acetone, benzene, butanol, chloroform, ethanol, ethyl acetate, hexane, methanol, toluene and distilled water for extraction, based on the polarity basis for the purpose of minimizing the process and error in identification and characterization of the active compound. Results: Regarding the percentage of yield, aqueous extract yielded the highest percentage, following that methanolic extract and other extracts in the order of ethanol, hexane, chloroform, ethyl acetate, butanol, benzene, acetone and toluene. In the perception of antibiotic activity, ethyl acetate and methanol showed better activity against both the species. Through the results, it is clear that the methanolic extract have mild antimicrobial activity. The antimicrobial activity in the form of zone of inhibition was below the value of 7.5 mm. Further, the qualitative analysis was carried out only for the methanolic extract, due to its overall activity. Through the qualitative analysis by thin layer chromatography it happened to know that 3 bands were formed and their R f values were 0.07, 0.17 and 0.44 respectively. Conclusions: The results showed that the solvent extracts of the species Thais bufo have no potential antibiotic activity, hence it cannot be considered for the further research in the field of drug discovery.

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