Antibiotic Resistance Patterns of Lactic Acid Bacteria Isolated From Different Fermented Milk Products of Turkish Origin
Journal Title: Microbiology Research Journal International - Year 2017, Vol 20, Issue 4
Abstract
Aims: Lactic acid bacteria isolated from 48 Turkish fermented milk products obtained from local markets, dairies or bazaars were investigated for their resistance of antibiotics including ampicillin, erythromycin, gentamicin, chloramphenicol, lincomycin, meropenem, ciprofloxacin, teicoplanin, tetracycline and vancomycin. Place and Duration of Study: Adnan Menderes University Biology Department, Microbiology laboratory, between 2012-2014 Methods: LAB strains, belonging to 14 species of Lactobacillus (n=68), 1 species of Lactococcus (n=16), 5 species of Enterococcus (n=14) and 2 species of Streptococcus (n=17) were isolated and identified at species level by their 16S rRNA gene sequencing. Minimal Inhibitory Concentrations (MIC) for 10 antibiotics were determined by agar dilution test using multipoint inoculator. Antibiotic resistance genes for erythromycin [erm(A), erm (B), erm (C)], gentamycin aac(6’) aph(2’’), chloramphenicol (cat), tetracycline [tet(K), tet(L), tet(M), tet(S), tet(Q)] and vancomycin [van(A), van(B), van(C), van(X)] were investigated in strains. Mating experiments were done with E. faecalis JH2-2 to detect the transferability of resistance genes. Results: Among 115 strains antibiotic resistance was detected against lincomycin (27,8%), tetracycline (20%), ampicillin (13,9%), meropenem (11,3%), gentamycin (10,4%), erythromycin (7,8%), ciprofloxacin (6,1%), chloramphenicol (3,4%), vancomycin (0,87%). While all these strains were susceptible to teicoplanin, 29,5% of isolates were multiple resistant to various antibiotics. The resistance genes aac(6’) aph(2’’), erm(B), tet(L), tet(M) and van(C) were detected in strains of, Lb. delbrueckii subsp. bulgaricus, Lb. kefiri, Lc. lactis subsp. lactis, S. lutetiensis, S. macedonicus, E. faecalis, E. gallinarum isolated from some cheeses and one household kefir samples. Conclusion: Antibiotic-resistant LAB carrying transeferable resistance genes in some Turkish dairy products, may act as a dangerous vehicle for transmission of these traits to the other bacteria by horizontal gene transfer.
Authors and Affiliations
Melihcan Ozteber, Gamze Başbülbül
Keywords
Related Articles
- EP ID EP316153
- DOI 10.9734/MRJI/2017/33221
- Views 83
- Downloads 0
Antibacterial Activity of Actinomycetes Isolated from Waste Dump Soil from Western Uganda
Aims: To determine the antibacterial activity of actinomycetes isolated from waste dump soil in Western Uganda. Study Design: The study was an experimental laboratory design. Place and Duration of Study: Waste dump soil...
Antimicrobial Susceptibility of Urinary Escherichia coli from Outpatients with Community Acquired Urinary Tract Infections, Report from Tertiary Health Care Center, Egypt
Background: Urinary tract infection (UTI) is one of the most common infections at the community setting. Escherichia coli are the main agent of UTIs. Antibacterial resistance spreads rapidly among community acquired urin...
An in-vitro Analysis of the Antioxidant and Antimicrobial Properties of the Methanol Extract of Hypoxis hemerocallideacorm (MEHHC) from Botswana
Aims: To evaluate the antimicrobial and antioxidant activity of the methanol extract of Hypoxis hemerocallidea corm (MEHHC) with different assays. Study Design: In vitro evaluation of antibacterial and antioxidant activi...
Molecular Characterization and Detection of Infection in Vector Snails of Urinary Schistosomiasis around Erinle and Eko Ende Dams in South West Nigeria
Aims: The prevalence of the schistosome cercariae in snail intermediate hosts has been known as one of the valuable predictors of the level of schistosomiasis transmission in different localities. This study was undertak...
Correlation of Virulence Determinants of Staphylococcus aureus to the Severity of Diabetic Foot Ulcers in a Tertiary Care Centre, Egypt
Aim: This study aimed to estimate the correlation of the Staphylococcus aureus virulence determinants to the severity of the diabetic foot ulcers in patients admitted to diabetic foot Unit at Mansoura University Hospital...