Antibiotic resistance, virulence factors, and phylogenetic groups of Escherichia coli isolated from hospital wastewater: A case study in the west of Iran
Journal Title: Environmental Health Engineering and Management Journal - Year 2023, Vol 10, Issue 2
Abstract
Background: The appropriate management of hospital wastewater is an essential process to prevent the spread of pathogenic strains of bacteria including Escherichia coli in this study, the antibiotic resistance, virulence characteristics, and phylogenetic diversity of E. coli isolated from the raw and treated hospital wastewater in a general hospital in the west of Iran were evaluated. Methods: E. coli isolates were recovered and identified using culture and biochemical tests. Sixty isolates were used and antimicrobial resistance, virulence genes, antibiotic-resistant genes (ARGs), and phylogeny groups of isolates were determined using polymerase chain reaction (PCR) assay. The antibiotic resistance was tested using disk diffusion. Results: The antibiotic susceptibility testing indicated that the resistance to co-trimoxazole was the most common, followed by ceftriaxone, amikacin, and gentamicin. Multi-drug resistance (MDR) was observed in 90% of raw and 96.66% of treated sewage isolates. The phylogeny groups B1 and A were the most common groups among isolates of raw and treated sewage, respectively. The most common virulence genes detected were sfa, papC, and fyuA; while pic and sepA genes were not found in the isolates. The most common ARGs were blaTEM (in 90% isolates of raw and 92.5% of treated sewage) and blaCTX-M (in 60% isolates of raw and 77.5% of treated sewage). The blaSHV-5 gene was not detected among isolates. Conclusion: The results highlight the potential of hospital wastewater as a source for spreading the virulent and multi drug-resistant strains of E. coli.
Authors and Affiliations
Arezoo Bozorgomid, Roya Chegene Lorestani, Mosayeb Rostamian, Fatemeh Nemati Zargaran , Zhila Shahvaisi- Zadeh, Alisha Akya
Keywords
Related Articles
- EP ID EP718079
- DOI 10.34172/EHEM.2023.15
- Views 15
- Downloads 0
A new magnetic bio-sorbent for arsenate removal from the contaminated water: Characterization, isotherms, and kinetics
Background: Arsenic (AS) is a heavy metal pollutant in water that has been known as one of the most important environmental contaminants due to its serious effects on both human health and the environment. This study w...
Evaluation of adsorption efficiency of activated carbon functionalized with methyl diethanolamine in carbon dioxide gas
Background: Adsorption is a process in which some of the components in the fluid phase, are selectively transferred to the surface of the porous solid particles in the filled bed, which is called the adsorbent. The aim...
Biological hydrogen production from synthetic wastewater by an anaerobic migrating blanket reactor: Artificial neural network (ANN) modeling
Background: This study aimed to evaluate an anaerobic migrating blanket reactor (AMBR) for biological hydrogen production, and also to investigate its capability to treat synthetic wastewater. Methods: A five-compartmen...
Seawater reverse osmosis membrane fouling causes in a full scale desalination plant; through the analysis of environmental issues: raw water quality
Background: Membrane clogging is one of the most important problem for desalination plant operators in Iran, therefore, this study was conducted to investigate the main causes of this problem using field analysis. Met...
Comparison of hybrid regression and multivariate regression in the regional flood frequency analysis: A case study in Khorasan Razavi province
Background: Magnitude, rate and frequency of the stochastic and unexpected events are of great significance and importance in hydrology. Nowadays, for economic planning of the projects, the use of analytical methods of u...