Antibiotic susceptibility and the presence of blaIMP and blaVIM genes among of Pseudomonas aeruginosa strains resistant or susceptible to imipenem

Journal Title: Progress in Health Sciences - Year 2012, Vol 2, Issue 1

Abstract

Introduction: Pseudomonas aeruginosa rods are increasingly causing serious infections in hospitalized patients. Particularly worrying is the increase of resistance to carbapenems antibiotics. Purpose: To evaluate susceptibility and the occurrence of genes (blaIMP and blaVIM) encoding resistance to carbapenems among Pseudomonas aeruginosa strains. Materials and methods: Studies were conducted for 50 strains of Pseudomonas aeruginosa (25 susceptible and 25 resistant to imipenem). Susceptibility to antibiotics was tested using the diffusion method and discs with antibiotics and / or strips with gradient concentrations of antibiotics. In the second phase we tested the ability of MBL production by all strains using the CD and DDST technique described in the literature. The next stage of the study was to evaluate the prevalence of carbapenems resistance genes. These studies were performed by PCR technique. Results: The studies found in both groups of Pseudomonas aeruginosa rods similar percentage of strains resistant to aminoglycoside antibiotics (from 72% to 88%) and ciprofloxacin (84%). There was no presence of the genes in any of the tested groups of Pseudomonas aeruginosa. Conclusion: Pseudomonas aeruginosa strains resistant to imipenem no posses blaIMP and blaVIM genes therefore their resistance was conditioned by the presence of other mechanisms. Antibiotics with high activity against Pseudomonas aeruginosa strains resistant to imipenem were polymyxin B (100% susceptible strains) and colistin (96% susceptible strains).

Authors and Affiliations

P. Sacha, D. Ojdana, J. Jaworowska, K. Kaczyńska, A. Bajguz, Tryniszewska

Keywords

Related Articles

Stigma of people living with HIV/AIDS

A specific character of HIV/AIDS has caused numerous complications either medical, legal or social. HIV infection exposes its carriers to frequent acts of intolerance or stigma by the healthy part of the population. Nega...

Most common health problems of elderly in the opinion of health sciences students in Poland, Belarus and Greece

Introduction: Health is one of the most important and cherished values in the elderly. It has been estimated that among people over 65 years old 80% of people suffer from at least one physical illness. Purpose: To identi...

Mental health and coping strategies among nursing staff in public health services

Purpose: The present study aimed to evaluate mental health and coping strategies among nursing staff in two public hospitals of Greece. Materials and methods: This cross-sectional study was conducted on 318 nurses workin...

The extent and manner of passing the information concerning the surgical implantation of cells, tissues or organs to the recipient of the transplant under the Polish law

The purpose of article is to present, analyse and evaluate the effective legal regulations concerning the extent and manner of passing information about surgical implantation of cells, tissues or an organ to the recipie...

Assessment of knowledge about obesity among students in a medical college in Kancheepuram district, Tamil Nadu

Introduction: Overweight and obesity are the fifth leading risk for global deaths. It is not only the degree of excess fat that is important, but also its distribution in the body that determines the health risks associa...

Download PDF file
  • EP ID EP163724
  • DOI -
  • Views 99
  • Downloads 0

How To Cite

P. Sacha, D. Ojdana, J. Jaworowska, K. Kaczyńska, A. Bajguz, Tryniszewska (2012). Antibiotic susceptibility and the presence of blaIMP and blaVIM genes among of Pseudomonas aeruginosa strains resistant or susceptible to imipenem. Progress in Health Sciences, 2(1), 12-18. https://europub.co.uk/articles/-A-163724